促进种子萌发的重楼内生菌的筛选.doc

促进种子萌发的重楼内生菌的筛选 宋发军于鹏飞张鹏*,刘佳席晓圆赵盛英 （中南民族大学 生命科学学院生物技术国家民委重点实验室/武陵山区特色资源植物种质保护与利用湖北省重点实验室，武汉 430074） 摘要 通过比较60株内生细菌发酵液的水相提取液和有机相提取液对冬小麦种子萌发的影响，发现株细菌的有机相提取物具有促萌发作用将4株细菌的发酵液依次用石油醚、氯仿、乙酸乙酯、正丁醇进行萃取，蒸干物用甲醇溶解并稀释成不同浓度处理冬小麦种子结果表明属74#菌的石油醚相提取物、属108#菌的氯仿相提取物、属138#菌的氯仿相提取物149#菌的乙酸乙酯相提取物的促萌作用较强；其中属138#菌的氯仿相提取物的1/2稀释液的促萌作用最强 关键词重楼内生菌；种子萌发；Q939.96 文献标识码 A 文章编号 1672-4321- Screening of Polyphylla Endophytes with Germination Effect Song Fajun , Yu Pengfei, Zhang Peng*, Liu Jia, Xi Xiaoyuan, Zhao Shengying （College of Life Sciences, South-Central University for NationalitiesNational Committee Key Laboratory for Biotechnology /Hubei Provincial Key Laboratory for Protection and Application of Special Plant Germplasm in Wuling Area of China ,Wuhan 430074, China） Abstract The effect of aqueous phase and organic phase of sixty fermentation broth of Polyphylla endophytic bacteria on the germination of winter wheat were compared, and the organic phases of 8 endophytes showed germination effect. The fermentation broth of 4 endophytic bacteria were then extracted by petroleum ether, chloroform, ethyl acetate and n-butanol, respectively. The dried product was dissolved with methanol and diluted into different concentration to treat winter wheat seeds. The results showed that the petroleum-ether extract from Enterobacter?sp. 74#, the chloroform extract from Acinetobacter sp. 108# , the chloroform extract from Bacillus sp. 138# and the ethyl acetate extract from Klebsiella sp. 149# possessed significant germination effect（p 0.05）. The half dilution of the chloroform extract from Bacillus sp. 138# had the best germination effect（p 0.01）. Key words Polyphylla endophyte; winter wheat seeds ; germination; extract 收稿日期 2014--07 通讯作者 *张鹏（1977-），男，副教授，研究方向：药用植物及其内生菌, E-mail?: zhangpenghust@126.com 作者简介 宋发军（1967-），男，教授，研究方向：应用生物化学, E-mail:songfajun@ 基金项目 国家自然科学基金资助项目，中南民族大学2014年硕士研究生学术创新基金2014sycxjj112) 植物内生菌是一类多样性丰富的微生物类群，常生活在健康植物的各种组织和器官内部的细胞或细胞间隙内，一般不引发宿主植物明显感染.早在1