Smad4条件基因敲出小鼠的前庭功能检测 - 第三军医大学学报.DOC

Smad4条件基因敲出小鼠的前庭功能检测 邓安春1，杨仕明2，黄德亮2，孙建和2，杨 晓3 （400037 重庆，第三军医大学新桥医院耳鼻咽喉科1；100853 北京，解放军总医院耳鼻咽喉头颈外科，解放军耳鼻咽喉研究所2；100071 北京，军事医学科学院牛物工程研究所发育和疾病遗传学研究室3） [摘要] 目的 通过设计的一系列前庭功能检测实验对同窝成组的软骨组织Smad4条件基因敲除野生型、杂合子、纯合子小鼠的前庭功能进行评估。方法 前庭功能检测方法：一般行为观察；空间姿势反射；游泳实验；前庭眼反射（VOR），使用前庭功能检查系统记录眼震电图；短声刺激前庭诱发肌源性电位（VEMP）国家计划Evaluation of vestibular functions in Smad4 conditional knockout mice Deng Anchun1,Yang Shiming2,Huang Deliang2,Sun Jianhe2,Yang Xiao3（1 Department of Otolaryngology, Xinqiao ospital, Third Military Medical University, Chongqing，400037；2 Department of Otolaryngology and Head Neck Surgery, Institute of Otolaryngology, Chinese PLA General Hospital, Beijing，100853Genetic Laboratory of Development and Diseases，Institute of Biotechnolog，Beijing，100） evaluate vestibular functions in Smad4 conditional knockout mice. Methods Vestibular functions were evaluated through series tests which consist of general behavioral observation, air righting reflex, swimming test, VOR (ENG was recorded using vestibular function examination system) and VEMP (Potentials evoked by loud clicks on the pre-vertebral muscle at the level of the third cervical vertebral bone were recorded using silver ball electrodes under general anesthesia). Results Behaviors, gaits, air righting reflex and swimming test in Smad4-/- mice were normal, and all the three genotypes mice can be induced identical nystagmus through rotation(P0.05). Latency of VEMP had no difference among the three genotypes mice(P0.05) but the response threshold of VEMP in Smad4-/- mice were obviously elevated(P0.01). Conclusion Only partial functions of saccule were damaged to some extent in Smad4 conditional knockout mice, and other vestibular endorgans had normal functions. [Key words] Smad4; conditional knockout; vestibular functions Supported by the National 863 program special projects (2007AA02Z150) , the National Natural Science Foundation of China (NSF30871398) ,the Scientific Research Foundation of Chongqing