乙烯的直接生物合成.pdf

孙芝兰 等/ 乙烯的直接生物合成 1431 生 物 工 程 学 报 Chinese Journal of Biotechnology October 25, 2013, 29(10): 1431−1440 /cjbcn ©2013 Chin J Biotech, All rights reserved 综 述 乙烯的直接生物合成 孙芝兰，陈以峰 江苏省农业科学院农业生物技术研究所 江苏省农业生物学重点实验室，江苏 南京 210014 孙芝兰, 陈以峰. 乙烯的直接生物合成. 生物工程学报, 2013, 29(10): 1431–1440. Sun ZL, Chen YF. Direct biosynthesis of ethylene. Chin J Biotech, 2013, 29(10): 1431–1440. 摘 要: 乙烯是世界上需求最大的化工原料，随着石油资源的日益枯竭和原油价格的不断攀升，生物乙烯迎来 了重大发展机遇。文中主要比较两条生物乙烯合成途径——生物乙醇脱水制备乙烯途径 ( 即间接途径) 和生物乙 烯的直接合成途径，重点论述了直接合成途径和途径中关键酶的性质、利用微生物直接合成生物乙烯的基因工程 策略、工程化制造生物乙烯的前景及成功事例，并指出直接合成生物乙烯替代石化乙烯具有较大的市场潜力。 关键词: 生物乙烯，直接合成，微生物，基因工程 Direct biosynthesis of ethylene Zhilan Sun, and Yifeng Chen Key Laboratory of Agrobiology of Jiangsu Province, Institute of Biotechnology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, Jiangsu, China Abstract: Ethylene is the most widely used petrochemical feedstock globally. The development of bio-ethylene is essential due to limited fossil fuels and rising oil prices. Bio-ethylene is produced primarily by the dehydration of ethanol, but can alternatively be directly produced from ethylene biosynthesis pathways in plants, algae, or microorganisms by using cheap and renewable substrates. This review addressed the biosynthesis of ethylene in plants and microorganisms, the characterization of key enzymes, genetic engineering strategies for ethylene biosynthesis in microorganisms, and evaluated its perspective and successful cases toward the industrial application. The direct production of bio-ethylene from