乙酰丁香酮在植物转基因研究中的作用 - 中国农学通报.pdf

中国农学通报 2011,27(05):292-299 Chinese Agricultural Science Bulletin 乙酰丁香酮在植物转基因研究中的作用 1 2 1 1 1 董喜才 ，杜建中 ，王安乐 ，魏国英 ，陈朝辉 1 2 （山西省农业科学院棉花研究所，山西运城044000 ；山西省农业科学院生物技术研究中心，太原030031） 摘 要：分析了从20 世纪80 年代到2007 年间大量的有关乙酰丁香酮对植物遗传转化的影响的文献，涉 及范围包括乙酰丁香酮对转化对象的影响方式、对转化率大小的影响以及与其它转化方法等的协同作 用等等。此文介绍了受转化对象的基因型、转化方法、加入时机、加入量以及培养基pH 值等对乙酰丁香 酮的作用效果的影响。得出乙酰丁香酮可提高转化频率、如酸性条件下更有利于乙酰丁香酮发挥作用 等结论。分析结果证明乙酰丁香酮在植物遗传转化中起着重要的作用。 关键词：乙酰丁香酮；植物；转化；转化率 中图分类号：Q81 文献标志码：A 论文编号：2010-3251 RoleofAcetosyringoneinPlantsTransformationResearcher 1 2 1 1 1 Dong Xicai , Du Jianzhong , Wang Anle , Wei Guoying , Chen Zhaohui 1 ( CottonResearchInsititute, ShanxiAgriculturalScienceAcademy, Yuncheng Shanxi 044000; 2 AgriculturalBiotechnologyResearchCenter, ShanxiAgriculturalScienceAcademy, Taiyuan 030031) Abstract: The papers has been published between the 1980’s and 2007 in relation to the effect of acetosyringone (AS) on plant genetic transformation, for example, the effect of AS on transformed plants and transformation frequency as well as the interaction between AS and transformation methods, were analysed. Here we discussed the effects on the action model of AS by genetypes of transformed objects, transformation methods, the time and dosage of AS which was added and the pH value of medium used for transformation. From that we can become a conclusion that AS could enhance transformation frequency, acidic cultures also favoured the function of AS, et a