介孔二氧化硅阿霉素纳米粒的制备及体外释放考察 - 中国现代应用药学.pdf

·药 剂· 介孔二氧化硅阿霉素纳米粒的制备及体外释放考察 徐爱仁 1,2 1,2* 1,2 1,2 1,2 ，马卫成 ，应景艳 ，何文跃 ，陈武 (1.宁波市泌尿肾病医院药剂科，浙江 宁波 315100 ；2.宁波大学，浙 江 宁波 315211) 摘要：目的 制备载阿霉素的介孔二氧化硅纳米粒(MSN) ，并对其体外释放进行初步研究。方法 通过聚合法制备MSN ， 应用透射电镜表征纳米粒的形态，动态光散射粒径测定仪测定粒子的平均粒径及分布。紫外可见分光光度法评价载药量、 包封率及体外释放。结果 纳米粒分布均一，平均粒径约70 nm(PDI0.1)。药物的载药量和包封率分别为(20.38 ±3.58)% 和(55.29 ±5.17)%。纳米粒经24 h 恒温振荡释放达平衡，在pH 5.5 磷酸盐缓冲液中累积释放分数达到95 ％。结论 MSN 具有较高的药物载药量，有望成为一种新型的化疗药物载体。 关键词：阿霉素；介孔二氧化硅纳米粒；体外释放 中图分类号：R943 文献标志码：B 文章编号：1007-7693(2012)10-0914-03 Preparation and in Vitro Release of Doxorubicin Loaded Mesoporous Silica Nanoparticles XU Airen1,2, MA Weicheng1,2*, YING Jingyan1,2, HE Wenyue1,2, Chen Wu1,2(1.Department of Pharmacy, Renopathy and Urology Hospital, Ningbo 315100, China; 2.Ningbo University, Ningbo 315211, China) ABSTRACT: OBJECTIVE To prepare doxorubicin-loaded mesoporous silica nanoparticles and evaluate its release characteristics in vitro. METHODS Mesoporous silica nanoparticles were achieved by condensation methods. The morphology was examined by transmission electron microscope and dynamic light scattering particle size analyzer. Doxorubicin loaded mesoporous silica nanoparticles were achieved by stirring. The encapsulation efficiency, drug loading rate and in vitro cumulative release were evaluated by UV spectrophotometry. RESULTS Distribution of nanoparticles was uniform. The average particle size was about 70 nm (PDI0.1). The drug loading rate and encapsulation efficiency were (20.38±3.58)% and (55.29±5.17)%. The delivery system reached release equilibrium after shaking in water bath for 24 h. The cumulative release proportion was above 95% in PBS(pH 5.5). CONCLUSION Mesoporous silica nanoparticles with high drug encapsulation efficiency can be used as the carrier of anticancer drug doxorubicin. KEY WORDS: doxorubicin; mesoporous silica nanoparticle; release in vi