全反式维甲酸对阿霉素肾病小鼠肾脏保护作用 - 第三军医大学学报.doc

全反式维甲酸对阿霉素肾病小鼠肾脏保护作用 王晓丽，崔晶晶，阳海平，刘 伟，李 秋 （400014重庆，重庆医科大学附属儿童医院临床免疫研究室） [摘要] 目的 探讨全反式维甲酸（all-trans retinoic acid ATRA）对阿霉素肾病模型小鼠的肾脏保护作用。方法 将30只BALB/c小鼠，分为正常对照组、阿霉素肾病模组（模型组）和阿霉素肾病全反式维甲酸ATRA治疗组（ATRA治疗组），分别腹腔注射给药10周。免疫组化分析肾脏凋亡蛋白Fas、FasL的表达水平，Real-time PCR技术检测肾脏Fas、FasL的 mRNA表达。结果 模型组与对照组比较，尿蛋白、血脂胆固醇（TC）（3.09±0.89）、甘油三酯（TG）（1.33±0.29）水平明显升高（P值均小于0.05）；ATRA治疗组与模型组比较TC、TG分别为（1.27±0.31）、（1.12±0.45）明显降低(P均小于0.05)。RT-PCR分析，模型组与正常组相比较，肾组织的Fas、FasL分别为（2.9537±1.5581）、（3.6806±0.27313）的表达明显升高（P0.05、P0.05）；ATRA治疗组与模型组相比较Fas、FasL分别为（0.6013±0.24993）、（0.7299±0.73044）明显增强（P0.05、P0.05）。结论 ATRA可能通过降低肾脏Fas、FasL的表达，而对肾脏起到保护作用。 [关键词] 阿霉素肾病；全反式维甲酸；凋亡； [中图法分类号] R725.7 [文献标志码] A Reno-protective Effect of All-trans Retinoic Acid on Adriamycin-induced Nephropathy Mice Wang Xiaoli，Cui Jingjing，Yang Haiping，Liu Wei，Qiu Li（Departtment of Clinical Immmunology，Childrens Hospital of Chongqing medical university，Chongqing 400014 China） [Abstract] Objective To investigate the reno-protective effect of All-trans Retinoic Acid on Adriamycin-induced Nephropathy mice. Methods BALB/C mice were randomly divided into normal control，ADR-induced nephropathy（model），and ATRA-treated（treatment）groups. After ADR-induced nephropathy establishment in model and treatment groups，and ten weeks of intraperitoneal injection administer of corn oil in normal control and model group and ATRA in treatment group. Expression of Fas、FasL was measured by immunohistochemistry and Real-time PCR. Result All-trans Retinoic Acid significantly decreased 24-h urinary protein excretion, serum TC（3.09±0.89） and TG（1.33±0.29）(P0.05、P0.05、P0.05). Immunohistochemistry and Real-time PCR analysis showed that Fas、FasL expression level（分别为0.6013±0.24993、0.7299±0.73044） were significantly high lower in treatment groups(P0.05、P0.05). Conclusions All-trans Retinoic Acid has anti-apoptosis on adriamycin induced nephropathy mice to protect kidney damage. [Key words] Adriamycin-induced Nephropathy；All-trans Retino