关节应力平衡失调对大鼠膝关节软骨退变的影响 - 中国实验动物学报.doc

研究报告 脊神经后根切断对大鼠膝关节软骨退变的影响 张 影1(?,林 毅1?,颜 赫1?,张才新1,时晓迪1?,金利新2 (1. 青岛大学医学院临床学院,青岛 266071；2. 青岛大学医学院解剖学教研室,青岛 266071) 【摘要】目的 观察脊神经后根切断大鼠不同时期关节软骨的变化特点及规律，进一步验证部分感觉障碍可以引起关节软骨损伤。方法 10周龄SPF级雄性Wistar大鼠33只随机分为实验组（18只）和对照组（15只）。切断实验组L3、L4脊神经后根。对照组只切开皮肤及椎旁肌。观察大鼠行为学改变。于术后2周6周10周HE染色、番红O/固绿染色观察关节软骨的组织学改变及特点。结果 术后随时间推移，实验组大鼠右侧后肢经历一过性瘫痪、协调运动障碍及主动运动的变化过程。右后肢股骨髌面逐渐变浅变宽。关节软骨依次出现表面粗糙、细胞排列紊乱、细胞减少、潮线复制、漂移和中断等变化。实验组关节软骨ACC/TAC值随时间推移呈逐渐增大（t=5.25~8.13，P0.05）。结论 脊神经后根切断可引起关节软骨损伤及退行性改变。 【关键词】软R684 【文献标识码】Effect of dorsal rhizotomy on cartilage of degenerative changes in knee joint of rats ZHANG Ying1,LIN Yi1,YAN He1,ZHANG Cai-xin1,SHI Xiao-di1,JIN Li-xin2 (1. Department of Clinical Medicne，Medical College of Qingdao University , Qingdao 266071, China ; 2. Department of Anatomy, Medical College of Qingdao University , Qingdao 266071, China) [Abstract] Objective To investigate the changing characteristics and rules of the different period of articular cartilage in rats after dorsal rhizotomy , and to verify the partial sensory disturbance can cause articular cartilage injury. Methods Thirty-three ten-month-old SPF male Wistar rats were randomly divided into experimental group (n=18) and control group (n=15). The rats in the experimental group were sectioned the L3、L4 dorsal roots in the right. The rats in the control group were only incised the skin and paravertebral muscles. To observe the behavior changes in rats. At 2, 6 and 10 weeks, the specimens of the right hind lower end of femur were drawn out to make paraffin sections, then HE staining and Safranin O/ Fast Green staining. The changes and characteristics of the morphology of the articular cartilage was observed. Results With the prolonging of period, the right hind limb of the experimental rats through the change process of transient paralysis, coordination of movement disorders and active movement. In the experimental group, the patellar surface of right hind femur gradually became shallow and wide. The articular cartilage und