左卡尼汀注射液对阿霉素所致心肌细胞损伤的保护作用.pdf

• 18 • 现代药物与临床 Drugs Clinic 第 28 卷 第 1 期 2013 年 1 月 左卡尼汀注射液对阿霉素所致心肌细胞损伤的保护作用 李雅静，王 晨* 天津医科大学附属肿瘤医院 天津市肿瘤防治重点实验室，天津 300060 摘 要：目的 观察左卡尼汀注射液对阿霉素所致的心肌细胞损伤的保护作用。方法 将培养 5 d 的新生Wistar 乳鼠心肌细 胞随机分为正常对照组、阿霉素 1 μg/mL 阳性对照组和左卡尼汀 10、20 、30 μg/mL 保护组，加药后继续培养 24 h ，测定细 胞存活率、乳酸脱氢酶（LDH ）释放量、超氧化物歧化酶（SOD ）活性和丙二醛（MDA ）含量。结果 左卡尼汀能显著提 高受损心肌细胞的存活率，抑制 LDH 释放量，减少 MDA 的生成，提高 SOD 活性。结论 左卡尼汀对阿霉素所致的心肌细 胞损伤具有保护作用。 关键词：左卡尼汀注射液；阿霉素；心肌细胞 中图分类号：R969 文献标志码：A 文章编号：1674 - 5515(2013)01 - 0018 - 03 DOI: 10.7501/j.issn.1674-5515.2013.01.005 Protection of Levocarnitine Injection on myocardial cell injury induced by adriamycin LI Ya-jing, WANG Chen Tianjin Key Laboratory of Tumor Prevention and Therapeutics, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, China Abstract: Objective To observe the protective effects of Levocarnitine Injection on myocardial cell injury induced by adriamycin. Methods From newborn Wistar neonatal rats, the cardiomyocytes cultured for 5 d were randomly divided into normal control group, adriamycin (1 μg/mL) positive control group, and levocarnitine (10, 20, and 30 μg/mL) protection group, then followed by being continuously cultured for 24 h after the administration. Cell survival rate, lactate dehydrogenase (LDH) release, superoxide dismutase (SOD) activity, and malondialdehyde (MDA) contents were detected. Results Levocarnitine could significantly improve the survival rate of the damaged myocardial cells, inhibit the release of LDH, decrease the level of MDA, and promote the activity of SOD. Conclusion Levocarnitine has protective effects on the myocardial cell injury induced by adriamycin. Key words: Levocarnitine Injection; adriamycin; myocardial cells 阿霉素属于蒽环类抗癌药，临床上广泛用于白 规格 10 mg/支，批号 070702 ），左卡尼汀注射液 （常 血病、淋巴瘤等肿瘤的治疗，但由于其具有心肌毒 州兰陵制药有限公司，规格 5 mL