肺癌干细胞与肺癌化疗药物敏感性的研究.doc

CD133和ALDH1在非小细胞肺癌表达与肺癌化疗药物敏感性关系的研究1 杨敏1 孙慧元1、2 柳红1 1徐州医学院病理教研室，江苏 徐州 221002；2浙江台州恩泽医疗中心台州医院病理科，浙江 台州 318000 摘要：目的 探讨肺癌干细胞标志物CD133和乙醛脱氢酶1( aldehydedehydrogenase1, ALDH1)与3种肺癌临床常用化疗药物顺铂（Cisplatin, DDP）、长春瑞滨（Vinorelbine, NVB）、吉西他滨（Gemcitabine, GEM）敏感性的关系。方法 采用链霉菌抗生物素蛋白-过氧化物酶连结法streptavidin-perosidase，SP60例NSCLC组织中CD133和ALDH1蛋白的表达情况，生物荧光抗癌药物敏感性检测试剂盒检测相应NSCLC病例肿瘤组织体外原代培养细胞对化疗药物DDP、NV及GCD133和ALDH1蛋白表达呈正相关（r=0.356，P0.05），CD133与ALDH1阳性共表达率（CD133+/ALDH1+）为48.33%。.CD133+的NSCLC组织对DDP、NVB和GEM的敏感率低于CD133-的NSCLC；ALDH1+的NSCLC组织对DDP、NVB和GEM的敏感率低于ALDH1-的NSCLC，差别均无统计学意义（P﹥0.05）3. CD133+/ALDH1+组对DDP、NVB和GEM的敏感率高于non-CD133+/ALDH1+组，差别有统计学意义（P﹤0.05论 肺癌干细胞标志物CD133和ALDH1可能在化疗药物耐药方面发挥着重要的作用，尤其是CD133+/ALDH1+以作为肺癌化疗药物敏感性的预测指标之一，可能成为肺癌耐药临床治疗的新靶点。NSCLC；CD133；ALDH1；Research of the relation between expressions of CD133 and ALDH1 and sensitivity rates of chemotherapeutic drugs in non-small cell lung carcinoma SUN Huiyuan1、2, LIU Hong1, YANG Ming1 1Department of Pathology, Xuzhou Medical College, Xuzhou 221002, China; Department of Pathology, 2Department of Pathology, Taizhou 318000, China Abstract: Objective To investigate the expressions of the two caner stem cell markers CD133 and aldehyde dehydrogenas1(ALDH1) and drug sensitivity of Cisplatin, Vinorelbine and Gemcitabine in 60 non-samll cell lung carcinoma(NSCLC). Methods The expression of CD133 and ALDH1 in 60 NSCLC were detected by immunohistochemistry of streptavidin-perosidase(SP); Tumor chemosensitivity assay kit was used to detect the sensitivity of chemotherapeutic drugs of DDP, NVB and GEM in the same case. Results 1.The expression of CD133 was positively correlated with the expression of ALDH1 in NSCLC with Pearson r=0.356, P0.05. 2. The sensitivity rates of chemotherapeutic drugs DDP, NVB and GEM in the group of CD133+was lower than the group of CD133-, as well as ALDH1. No significant correlation was found between the expression of CD133 /ALDH1 and sensitivity rates of chemotherapeutic drugs.3. The sensitivity rates of chemothera