脂质体阿霉素和热疗对脑胶质瘤细胞的影响.pdf

Med J Chin PLA, Vol. 38, No. 3, March 1, 2013 201 SWO-38 + +MTT ++ 80.16%3.78%62.09% 3.05% (40.27%2.32%)(30.56%2.03%) (16.51%1.26%)(P0.05) + + (P0.05) ++ 84.19% 2.69% 60.29% 1.47%(46.72%2.09%) (35.09%1.46%) (17.85%0.78%) (P0.05)++ (P0.05)SWO-38 R739.410.53 A 0577-7402(2013)03-0201-03 Effects of liposome-adriamycin (L-ADM) and thermotherapy on glioma cells: an experimental study 1 2* SHI Zheng-hua , ZHANG Jian-ning 1Department of Neurosurgery, Xijing Hospital, Fourth Military Medical University, Xian 710032, China 2 Department of Neurosurgery, Navy General Hospital, Beijing 100048, China * Corresponding author, E-mail: jnhang2005@ [Abstract]Objective To observe the effects of liposome-adriamycin (L -ADM) and thermotherapy on proliferation and apoptosis of SWO-38 glioma cells. MethodsThe SWO-38 glioma cells were cultivated in vitro. The effects of thermotherapy (43 ), ADM chemotherapy, L-ADM chemotherapy, thermotherapy + ADM chemotherapy, and thermotherapy + L-ADM chemotherapy on the cell proliferation and apoptosis were observed. The working concentration of ADM and L-ADM, and the cell proliferation rate were determined by MTT method. The apoptotic rate was determined by flow cytometry. ResultsThe proliferation inhibition rate of thermotherapy + L-ADM chemotherapy and thermotherapy + ADM chemotherapy was 80.16% 3.78% and 62.09% 3.05%, respectively, and it was significantly hi gher than that of L -ADM chemotherapy (40.27% 2.32%), ADM chemotherapy (30.56%2.03%) or thermotherapy (16.51% 1.26%, P0.05), and the pr