HBV-M定量及HBV-DNA检测相关性探究.docVIP

HBV-M定量及HBV-DNA检测相关性探究摘要：目的：探讨HBV-M定量与HBV-DNA检测的相关性。方法 ：采用时间分辨荧光定量法（TR FIA）和荧光定量PCR（FQ-PCR）法对360例疑似乙肝患者进行HBV-DNA和HBV-M定量检测。 结 果：HBeAg（+）组患者血清HBVDNA检?出率为95.17%，平均拷贝数为106.53±1.45 /ml， HBeAg（-）组患者血清HBV-DNA检出率为53.49%，平均拷贝数为104.52±1.60/ml，H BeAg（+）组的 HBV-DNA阳性率，拷贝数明显高于HBeAg（-）组（P＜0.05， P＜0.01）。HBsAg（ -）组患者仍有HBV-DNA阳性者。结论：单凭血清学标志物模式难以判断HBV的复制程度及传 染性强弱，定量检测HBV-DNA能真实反映HBV复制情况，对乙型肝炎的诊断及防治提供客观 依据。 关键词：乙型肝炎；时间分辨荧光法；荧光定量PCR；HBV-DNA；HBV-M 中图分类号： R512.62;R446.62 文献标识码： A 文章编号： 1008-2409(2008)05-0901-02 The study of the relationship between HBV-DNA and HBV-M concentration／OU Yang -qing, LIU Jian∥Departement of Laboratory,The Affiliated Hospital of Guilin Medical College, Guilin 541001, China Abstract: Objective: To explore the relatimship between the quantitative detection of HBV -DNA andHBV-M.Methods: HBV-DNA was detected by fluorescent quantitative PCR(FQ-PCR)a nd HBV-Mwas detected with TRFIA in serum samples from 360 patients who suspected casesof hepatitisB. Results: The detecting rate of HBV-DNA was 95.17% and the mean copy numberwas106.53±1.45/ml in patients of HBeAg(+) group, the detecting rate of HBV - DNA was 53.49%， and the mean copy number was 10452±1.60/ml. In patientsofHBeAg(-) group, the HBV-DNA positive rate and copy quantity in HBeAg(+) groupwas obviously high than that in HBeAg(-) group(P＜0.05 P＜0.01). HBV-DN A positivity may appear inHBsAg(-) group. Conclusion:Serum marker model only applied to judge the degreeof HBV replication and its infectivity were considerable diffiecult, Quantita-tively determination of the level of HBV-DNA can genuinely reflect the resultso f HBV replication and it is also of great significance for diagnosis and observa tion on the results in treatment of HBV patients.? Key words:hepatitis B;TRFIA;FQ-PCR;HBV-DNA;HBV-M 乙型肝炎是我国最常见的传染病之一，而乙型肝炎病者感染的诊断主要依赖于血清特异性抗 原、抗体和HBV-DNA检测，为探讨HBV感染者不同血清学标志物模式与HBV-DNA 定量的关系，笔者采用时间分辨和荧光定量PCR两种方法，对360 例疑似乙肝患者的血清进行检测，现将结果报告如下。 1 材料与方法 1.1 对象 360例均为2007年1月至2007年3月到我院就诊的所有同时做过乙肝两对半及HBV