MMP-2-COX-2及VEGF在膀胱移行细胞癌中表达及临床意义.docVIP

MMP-2\COX-2及VEGF在膀胱移行细胞癌中表达及临床意义[摘要] 目的探讨环氧化酶-2(COX-2)、金属基质蛋白酶-2(MMP-2)与血管内皮生长因子(VEGF)在膀胱移行细胞癌中的表达及临床意义。方法采用免疫组化SP法和高清晰度彩色医学图文分析系统对38例膀胱移行细胞癌和15例正常膀胱黏膜中COX-2,MMP-2,VEGF的表达进行检测分析。结果COX-2、MMP-2在膀胱癌中呈高表达,且随分期、分级的增高而表达增强;VEGF在膀胱癌中亦呈高表达,且与分期呈正相关,但与分级不相关。结论COX-2、MMP-2和VEGF参与膀胱癌侵袭转移,在膀胱移行细胞癌中的表达对其诊断及预后判断具有参考价值。 [关键词] 膀胱移行细胞癌;免疫组织化学;金属基质蛋白酶-2(MMP-2);血管内皮生长因子(VEGF);环氧化酶-2(COX-2) [中图分类号] R737.14[文献标识码] A[文章编号] 1673-9701(2010)10-12-03 Expression and Significance of MMP-2,COX-2 and VEGF in Bladder Transitional Cell Carcinoma XING TaoLIU HongyaoYAN PengWANG Zhihua Department of Urology,the First Hospital of Shanxi Medical University,Taiyuan 030001,China [Abstract] Objective To investigate the expression of cyclooxygenase-2(COX-2),matrix metalloproteinase-2(MMP-2)and vascular endothelial growth factor(VEGF) in human bladder transitional cell carcinoma and discuss their clinical significance. Methods BY using immunohistochemical stains and BI-2000 analysis system,the expression of COX-2,MMP-2 and VEGF in 38 cases of bladder carcinoma(as tumor group) and their expression in 15 cases of normal bladder(as control group)were analyzed. Results In the tumor group,the expression of COX-2 and MMP-2 was higher and their expression rates increased with the increase in the staging and grading. VEGF was also higher and its expression rates increased with the increase in the staging but not with the increase in the grading. Conclusion COX-2,MMP-2 and VEGF play an important role in the invasion step of bladder transitional cell carcinoma. The detection of their expressions may become a new approach to the diagnosis of urothelial carcinomas. [Key words] Bladder transitional cell carcinoma;Immunohistochemitry;Matrix metalloproteinase-2;Vascular endothelial growth factor;Cyclooxygenase-2 膀胱肿瘤是泌尿系统最常见的恶性肿瘤,其中膀胱移行上皮细胞癌占90%以上,其生物学特性是多中心、易复发及浸润性生长。复发后肿瘤恶性度可增高,浸润、转移等行为有增强趋势。此过程与肿瘤细胞外基质的降解、新生血管的大量形成有密切关系。大量研究表明环氧化酶-2(cyclooxygenase-2,COX-2)不仅通过促进细胞增殖、抑制凋亡参与多种肿瘤的发生过程,而且利用不同的途径来促进肿