MTT法对大肠癌药敏实验及临床应用探究.docVIP

MTT法对大肠癌药敏实验及临床应用探究[摘要] 目的 探讨用MTT法测定大肠癌细胞对化疗药物体外敏感性，研究大肠癌药敏的异质性和个体化疗的可行性。方法 用MTT法检测40份大肠癌标本对氟尿嘧啶（5-FU）、奥沙利铂（L-OHP）、伊立替康（CPT）单药应用、两药及三药（全量或半量）联合应用的敏感性。结果 不同标本的药物敏感性存在明显差异，单药中最有效的药物依次为伊立替康、奥沙利铂和氟尿嘧啶，敏感率分别为35.0%、27.5%和20.0%；两药联合应用均优于各自单药的抑制效果（P＜0.05），伊立替康联合奥沙利铂弱于伊立替康或奥沙利铂与氟尿嘧啶联合的抑制效果（P＜0.05）；三药联合应用的抑制效果显著优于两药联合（P＜0.05），三药全量及半量联合应用效果差异无统计学意义（P＞0.05）。结论 大肠癌对抗癌药物的敏感程度普遍较低，且存在明显异质性。试验结果与临床治疗经验比较一致，MTT可用于为大肠癌患者选择合适的化疗药物。由氟尿嘧啶、奥沙利铂及伊立替康的联合应用具有高效协同抑制大肠癌细胞的作用，可为临床难治性和复发性大肠癌患者的化疗提供参考。 [关键词] MTT法；大肠癌；药物敏感性 [中图分类号] R446.1 [文献标识码] B [文章编号] 1673-9701（2011）22-26-03 Clinical Application and Evaluation of Chemosensitivity of Human Colorectal Cancer Determined by MTT Assay in Vitro YANG Leilei1 DAI Yuechu2 DONG Milian2 YE Pubo1 LIAO Wei1 MEI Tong1 1.Wenzhou Medical College，Wenzhou 325000，China；2.Affilicated Taizhou Hospital of Wenzhou Medical College，Linhai 317000，China [Abstract] Objective To investigate the heterogeneity of chemo sensitivity in colorectal cancer using an MTT assay and the feasibility of individual chemotherapy. Methods The growth-inhibition effects of irinotecan（CPT），oxaliplatin（L-OHP） and fluorouracil（5-FU），single drug，two or three drugs on 40 human colorectal carcinoma cells were observed by MTT assay. Results There were considerable differences in chemo sensitivity between individuals. The most active single drugs in the assay was identified as irinotecan（CPT），oxaliplatin（L-OHP）and fluorouracil（5-FU）；35.0%，27.5% and 20.0% of specimens showed sensitivity to them，The inhibitory rates of any two drugs were significantly higher than those in single drug group（P＜0.05）．The growth-inhibition effect of CPT combined with L-OHP was lower than that of CPT or L-OHP combined with 5-Fu（P＜0.05）. The growth-inhibition effect of the three drugs combination was significantly enhanced compared with regiment of two drugs combination chemotherapy（P＜0.05）． Furthermore，whole-dose and half-dose combination of 3 drugs reached similar cell growth inhibitory rate（P＞0.05）.