Photofrin对小鼠光毒性病理学观察.docVIP

Photofrin对小鼠光毒性病理学观察[摘要] 目的：观察Photofrin对小鼠光毒性的病理学改变。方法：将100只小鼠随机分为5组，其中3组为Photofrin光照组，2组为对照组。Photofrin光照组小鼠分别尾静脉注射10 mg/kg、5 mg/kg和2.5 mg/kg的Photofrin ，对照组小鼠尾静脉注射5%葡萄糖注射液，模拟日光照射，分别于给药后24 h和第15天对背部去毛区、耳朵、尾巴皮肤，肝脏、肾脏等进行病理组织学检查。结果：Photofrin光照组小鼠耳朵皮肤可见明显的鳞状上皮增生，其中Photofrin 10 mg/kg光照组背部去毛区皮肤表面可见鳞状上皮乳头状增生、炎细胞聚集。结论：Photofrin能增强小鼠皮肤光毒性的损伤。 [关键词] Photofrin；光毒性；病理变化；肿瘤 [中图分类号]R991 [文献标识码]B[文章编号]1673-7210（2007）08（b）-103-02 Pathomorphology change of mice with the phototoxicity by Photofrin FU Xin-lu,WENG Yin-biao,LIANG Jin-qiang （Guangzhou Pharmaceutical Industrial Research Institute-The State Key Lab. of Safety Evaluation on New Drug in Guangzhou,Guangzhou 510240，China） [Abstract] Objective：To observe the pathomorphology change of mice with the phototoxicity by Photofrin. Methods：To administrate the drug-treated group micewith Photofrin 10 mg/kg、5 mg/kg、2.5 mg/kg by tail vein injection and the control group 5% glucose solution.At the second day and the fifteenth day，the back exposed skin，ear skin，tail，liver and kidney of mice were sampled to observe the pathomorphology change after the mice were exposed to the simulated sunlight.Results：The skin of mouse ear took on an obvious proliferation of the swuamous epithelium，the back skin of administration Photofrin 10 mg/kg mice took on an obvious papillary proliferation of the swuamous epithelium and the conglomeration of the proinflammatory cells on the skin surface.Conclusion：Photofrin enhances the phototoxicity on the mice. [Key words]Photofrin;Phototoxicity;Pathomorphology change;Tumor Photofrin (porfimer sodium)是一种光动学抗癌剂，含有酯醚联体的多卟啉低聚物，为光的增敏剂。Photofirn在肿瘤组织中的浓度比周围组织高，在适当波长光线的照射下，光敏剂吸收光能，在氧化剂存在的环境中，直接杀死肿瘤细胞，或者破坏肿瘤组织的脉管系统，导致瘤细胞死亡。Photofrin的全身毒性比较小，但是Photofrin 容易在皮肤中蓄积，其在小鼠皮肤中的半衰期至少72 h[1]，容易导致皮肤光毒性的发生。本试验通过病理学观察，评价Photofrin对小鼠皮肤组织的光毒性作用。 1 材料与方法[2~5] 1.1 动物与分组 SPF级NIH小鼠100只，由广东省医学实验动物中心提供，实验动物生产许可证号：SCXK（粤）2003-0002，实验动物质量合格证号:粤检证字2006A017。将动物随机分为5组，分别为Photofrin 10 mg/kg光照组、Photofrin 5 mg/kg光照组、Ph