δ阿片受体激动剂DADLE对脓毒症大鼠血浆高迁移率族蛋白1水平影响.docVIP

δ阿片受体激动剂DADLE对脓毒症大鼠血浆高迁移率族蛋白1水平影响[摘要]目的 研究δ阿片受体激动剂DADLE（D-Ala2-D-Leu5-enkephalin）对脓毒症大鼠血浆高迁移率族蛋白1（HMGB1）水平的影响。方法 将96只Sprague-Dawley （SD）大鼠分为假手术组（SO）、脓毒症组（SEP）、DADLE组[制模后立即给药DADLE（5mg/kg，腹腔内注）]， 每组32只。采用改良盲肠结扎穿孔方法（CLP）建立大鼠脓毒症模型，于制模后6h，12h，18h，24h每组各取8只处死取材。ELISA法检测血浆HMGB1水平。结果 12h 时SEP组和DADLE组HMGB1水平与SO组相比均明显升高（P＜0.05），且DADLE组明显低于SEP组（P＜0.05）；18h时，SEP组和DADLE组HMGB1水平达到最高。同样DADLE组明显低于SEP组（P＜0.05）；24h时SEP组和DADLE组HMGB1水平较18h有所降低，但DADLE组仍明显低于SEP组（P＜0.05）。结论 DADLE能明显降低脓毒症大鼠血浆HMGB1水平。 [关键词] DADLE；脓毒症；高迁移率族蛋白1 [中图分类号] R631 [文献标识码] B [文章编号] 1673-9701（2011）24-17-02 Effect of Delta-opioid Receptor Agonist on Serum HMGB1 Level in Rat with Sepsis WANG Ping TANG Chengwu FENG Wenming BAO Ying ZHU Ming Department of Hepatobiliary Surgery and Molecular Surgery，the First Peoples Hospital Affiliated to Huzhou University Medical College，Huzhou 313000，China [Abstract] Objective To study the effect of delta-opioid receptor agonist（DADLE） on serum HMGB1 level in rat with sepsis. Methods All 96 SD rats were randomly divided into sham operated group （SO），septic group（SEP）and DADLE group（DADLE）.Septic model was produced by cecal ligation and puncture（CLP）. In SO group，the abdomen was opened without any other treatment.In DADLE group，DADLE was administered at a dose of 5mL/kg by intraperitoneal injection after CLP. Rats were sacrificed at 6h，12h， 18h and 24h after CLP. HMGB1 level was determined by ELISA. Results The serum HMGB1 levels of SEP and DADLE groups began to increase at 12h，and reached the peak around 18h after CLP. Notably， serum HMGB1 levels of DADLE group at 12h，18h and 24h were significantly lower than those of SEP group（P＜0.05）. Conclusion DADLE could significantly decrease serum HMGB1 level of septic rat. [Key words] DADLE；Sepsis；CLP；High-mobility Group box 1 Protein 脓毒症是严重感染、创伤及大手术等应激状况时常见的并发症，已经成为当今重症监护室的首要死亡原因[1]。脓毒症时多种炎症因子参与了脓毒症的病理过程，高迁移率族蛋白B1（high mobility group box1 protein，HMGB1）作为潜在晚期炎性因子，是脓毒症致死效应的重要炎性介质，干预HMGB1可有效防止脓毒症和MODS的发生与发展[2，3]。δ阿片受体激动剂DA