中华大蟾蜍抗菌肽对癌细胞生长抑制作用.doc

中华大蟾蜍抗菌肽对癌细胞生长抑制作用[摘要] 目的 筛选中华大蟾蜍抗肿瘤活性物质，检测其抗肿瘤活性。方法 本实验通过凝胶过滤层析及高效液相色谱法，从皮肤分泌物中分离出具有抗细菌、抗肿瘤活性的物质，即中华大蟾蜍抗菌肽。以大肠癌细胞为体外实验模型，通过细胞形态学、MTT实验，研究中华大蟾蜍抗菌肽对SW480细胞的杀伤和生长抑制作用。结果 经过分离纯化得到较纯的活性物质，即中国大蟾蜍抗菌肽。通过细胞形态学观察到中华大蟾蜍抗菌肽作用组中细胞的数量明显的减少，细胞明显皱缩，成圆形，死细胞增多。MTT实验表明，中华大蟾蜍抗菌肽对SW480的生长抑制作用随着浓度的增加而加强。结论 本实验成功筛选到中华大蟾蜍抗菌肽，并检测其在体外对SW480具有抑制生长作用。 [关键词] 中华大蟾蜍；抗菌肽；生长抑制 [中图分类号] R73-34 [文献标识码] B [文章编号] 1673-9701（2011）36-24-03 Growth Inhibiting Effect of Antibacterial Peptides in Bufo Gargarizans on Cancer Cells CHEN Liping HE Lili BA Yunfei Department of Neurology，the People’s Hospital of Dehui City in Jilin Province，Dehui 130300，China [Abstract] Objective To screen the Bufo bufo gargarizans cantor active materials，and test its antitumor activity. Methods The Bufo bufo gargarizans cantor-antimicrobial peptides was separated and purified by gel filtration chromatography and HPLC. The colorectal cancer SW480 was used as the model in vitro. The cell morphology and MTT were accepted to study the lethal effect and growth inhibiting of Bufo bufo gargarizans cantor-antimicrobial peptides to SW480. Results It indicated that a species of Bufo bufo gargarizans cantor-antimicrobial peptides was got through gel filtration chromatography and HPLC. The number of cells reduced，and the shap of the cells become shrinkage round significantly treated by Bufo bufo gargarizans cantor-antimicrobial peptides. The more Bufo bufo gargarizans cantor-antimicrobial peptides added，the more lethal effect and growth inhibiting effection was showed up by MTT. Conclusion The experiment is successful in screening Bufo bufo gargarizans cantor-antimicrobial peptides，and testing the role of inhibition of growth to SW480 in vitro. [Key words] Bufo bufo gargarizans cantor；Antimicrobial peptide；Growth inhibiting 抗菌肽（antimicrobial peptides，AMPs）是生物免疫系统产生的一类抵抗外界病原体感染的多肽，广泛存在于昆虫、植物、动物及人体内。抗菌肽是一种小肽，分子量一般小于10kDa，仅仅包含12～50个氨基酸，由基因编码、核糖体合成[1-3]。抗菌肽一般可以形成α-螺旋和β-折叠，抗菌肽的α-螺旋结构具有完美的水脂两亲性[4]，此结构是决定抗菌肽抗菌活性的基础。它除有非特异性的抗细