六味地黄汤对D-半乳糖致衰老小鼠运动能力影响.doc

六味地黄汤对D-半乳糖致衰老小鼠运动能力影响[摘要] 目的：探讨六味地黄汤对Ｄ-半乳糖致衰老小鼠的运动能力影响机制。方法：以Ｄ-半乳糖致亚急性衰老小鼠为模型,同时给予六味地黄汤治疗,6周后以全面观察六味地黄汤对运动能力的影响及机制。结果：六味地黄汤能有效延长衰老小鼠的游泳力竭时间、提高衰老小鼠的学习记忆成绩,不同程度提高衰老小鼠心、脑、骨骼肌中的SOD、Ｎa+- K+-ATP、Ca2+-Mg2+-ATP酶活性,降低心肌细胞、骨骼肌细胞内Ca2+、ＭＤＡ含量。结论：六味地黄汤能有效防止Ｄ-半乳糖致衰老小鼠的运动能力下降，学习记忆障碍等多项衰老体征的出现。 [关键词] 六味地黄汤；Ｄ-半乳糖；衰老；运动；抗氧化酶；脂质过氧化物 [中图分类号] R33[文献标识码]A [文章编号]1673-7210（2008）04（b）-016-03 The influence of Liuwei Dihuang Decoction on the exercise performance of the aged mice caused by D-galactose LIU Ping1,2, DING Yu-qin2,WANG Ai-mei2 ,HU Qi-sheng1* (1.Department of Physiology Basic Medical College, Wuhan University, Wuhan 430071，China；2. Department of Physiology，Nanyang Medical College,Nanyang 473000，China) [Abstract] Objective: To observe the effects of Liuwei Dihuang Decoction on the exercise performance of the aged mice caused by D-galactose.Methods:Researches had been done which used the aged mice as models and gave them treatment of Liuwei Dihuang Decoction.Results:It was discovered that Liuwei Dihuang Decoction could effectively prolong the time of exhaustive swimming, improve the learning and memory achievements of the aged mice and the activity of SOD,Na+-K+-ATPenzyme, Ca2+-Mg2+-ATP enzyme in the aged mice’s heart, brain and skeletal muscles in different degrees and reduce the amount of the Ca2+, MDA in the muscular cells of the heart and skeleton.Conclusion: It is concluded that Liuwei Dihuang Decoction can effectively prevent the decrease of exercise performance, the obstruction of learning and memory of aged mice caused by D-galactose. [Key words] Liuwei Dihuang Decoction;D-galactose; Aged; Excercise; Antioxidant enzymes; LPO 六味地黄汤是平补肾阴的代表方剂，由熟地、山萸肉、山药、丹皮、泽泻、茯苓组成[1]，临床应用广泛，为历代医家所推崇。现代医学研究证明，六味地黄汤具有调节免疫，抗衰老，抗肿瘤，保肝，降血脂、血糖等作用。但六味地黄汤对肌肉的代谢和运动的影响尚未见报道。笔者采用Ｄ-半乳糖致衰老模型小鼠研究了六味地黄汤对心脏、脑、骨骼肌中的超氧化物歧化酶（Superoxide Dismutase SOD)、Ca2+-Mg2+-ATPase、Ｎa+-K+-ATPase活性、脂质过氧化的终产物丙二醛（Maleic Dialdehyde，MDA）含量，Ca2+浓度，结构变化，多项衰老体征指标的影响,同时测定动物运动能力的变化，旨在观察六味地黄汤对抗骨骼肌衰老，增强运动能力的作用。 1