哮喘患者血清肿瘤坏死因子α水平及肺功能相关性探究.doc

哮喘患者血清肿瘤坏死因子α水平及肺功能相关性探究[摘要] 目的：探讨哮喘患者血清肿瘤坏死因子α（TNF-α）水平变化对肺通气功能的影响。方法：正常人18例（A组）、哮喘患者急性发作期16例 （B组）及缓解期16例（C组）分别抽取其静脉血以酶联免疫吸附法（ELISA）测定血清TNF-α水平，并测定其与肺功能指标的相关性，结果进行统计分析。结果：三组间血清TNF-α水平有极显著性差异（F为26.27，P＜0.001?。q检验结果：B组患者血清TNF-α水平显著高于A组和C组，有极显著性差异（q为6.378，3.654；P＜0.001?，A组与C组比较，有非常显著性差异（q为5.875，P＜0.01?。肺功能指标与血清TNF-α的相关性分析结果显示哮喘患者血清TNF-α水平与FEV1%、FEV1/FVC呈负性相关（r值分别为-0.83，-0.69；均P＜0.05?，但其与Vmax25 和Vmax50则无相关关系(r值分别为-0.27，-0.31；P＞0.05)。结论：前炎性细胞因子TNF-α在哮喘急性发作期参与气道炎症的形成和肺通气功能的下降；在哮喘缓解期，其作用在于参与气道炎症的维持。 [关键词]哮喘；肿瘤坏死因子α；肺功能 [中图分类号] R394 [文献标识码]B [文章编号]1673-7210（2008）01（b）-033-03 Correlation study between the serum tumor necrosis factor-α level and pulmonary function in asthma WANG Ying (Department of Respiratory Medicine, Liaoyang Third Hospital, Liaoyang 111000, China) [Abstract] Objective:To evaluate the effects of the serum tumor necrosis factor alpha( TNF-α)level on pulmonary ventilation function in asthma.Methods:All subjects were divided into three groups, including A( 18 healthy volunteers as controls), B(16 cases of bronchial asthma attacks)and C(16 cases in stable conditions).Serum TNF-α levels were measured by ELISA. Statistical sinificance was analysed using analysis of variance and Student New-Keuls test and Duncan’s new multiple range method, we also assayed the correlation between TNF-α and pulmonary ventilation function.Results: The activity of TNF-α in group B was sinificantly higher than those of group A (q’= 6.378, P0.05 ) and Vmax50( r =-0.31,P0.05 ). Conclusion:Proinflammatory cytokine TNF-α plays important roles in the formation of airway inflammation and descent of pulmonary ventilation function in acute phase in asthma, and it takes part in the maintenance of airway inflammation and damage of small airway function in stable phase. [Key words] Asthma; Tumor necrosis factor-α; Pulmonary function 支气管哮喘（以下简称哮喘）是一种多种细胞特别是肥大细胞、嗜酸粒细胞、T淋巴细胞、嗜中性粒细胞、气道上皮细胞及多种细胞组分参与的慢性气道炎症性疾病，其主要特征是气道炎症和气道高反应性(AHR)。参与哮喘气道炎症的细胞因子有很多，其中肿瘤坏死因子α（TNF-α）是一种具有广泛活性的