三溴偶氮氯膦褪色分光光度法测定顺铂注射液的含量-中国现代应用药学.PDF

三溴偶氮氯膦褪色分光光度法测定顺铂注射液的含量 吴越1 2 2 2 ，颜冬梅 ，屠凌岚 ，郑晓亮 (1.浙江省人民医院药剂科，杭州 310014 ；2.浙江省医学科学院药物研究所，杭州 310013) 摘要：目的 建立催化动力学分光光度法测定常用抗肿瘤药顺铂注射液含量的方法。方法 在硫酸介质中，进行顺铂催 化溴酸钾氧化三溴偶氮氯膦的褪色反应，褪色程度与顺铂含量呈线性关系，利用催化动力学分光光度法进行测定。结果 顺铂-三溴偶氮氯膦- 溴酸钾反应液的最大吸收峰在 530 nm ，3~24 ng·mL1 内线性关系良好(r=0.999 1) ，平均回收率为 99.3% ，RSD 为1.24%(n=3) 。结论 本方法灵敏度高、操作简便快速，可用于顺铂注射剂的含量测定。 关键词：顺铂注射液；三溴偶氮氯膦；催化分光光度法；含量测定 中图分类号：R927.2 ；R917.102 文献标志码：B 文章编号：1007-7693(2011)06-0558-03 Catalytic Spectrophotometric Determination of Trace Cisplatin Injection by Tribromochlorophosphonazo 1 2 2 2 WU Yue , YAN Dongmei , TU Linglan , ZHENG Xiaoliang (1.Zhejiang Provincial People’s Hospital, Hangzhou 310014, China; 2.Zhejiang Academy of Medical Sciences, Hangzhou 310013, China) ABSTRACT: OBJECTIVE To establish a catalytic spectrophotometric method for determination of trace cisplatin injection. METHODS A catalytic spectrophotometric method based on the catalytic effect of platinum on the oxidation of tribromochlorophosphonazo [3-(2, 4, 6-tribromophenylazo)-6-(4-chloro-2-phosponophenylazo)-4,5-dihydoxynaphthalene-2,7- disufonic acid] with KBrO3 in acidic medium had been proposed for the determination of trace cisplatin. The fading reaction was performed directly in an aqueous solution and the optimal conditions were given. RESULTS The calibration curves were linear over the concentration range of 324 ng·mL1 and the average recoveries of cisplatin were 99.3%, the RSD was 1.24%(n=3). CONCLUSION The catalytic spectrophotometric method is simple, rapid and sensitive, and could be used for the determination of cisplatin injection. KEY WORDS: cisplatin injection; tribromochlorophosphonazo; catalytic spectrophotometer; con