叶绿素含量的测定方法主要有分光光度法-南京林业大学学报.PDF

光纤SPR传感器测定叶绿素含量的研究 封维忠，韩 艳，杨 静，张 冲 (南京林业大学信息科学技术学院，江苏 南京 210037) 摘 要：介绍了光纤SPR传感器的基本原理、检测系统，应用光纤SPR传感器与光度计测定了叶绿素含量。 结果表明．在检测条件相同情况下，光纤SPR传感器测定叶绿素含量具有较好的重复性与稳定性。建立了光 纤SPR传感器的共振波长与叶绿素含量的关系，可通过光纤SPR传感器快速、准确地测定叶绿素含量。 关键词：表面等离子体共振；共振波长；折射率；叶绿素 中图分类 号 ：TN253；TP212．14 文献标识码 ：A 文 章编号 ：1000-2006(2006)04-0021-04 StudyontheContentofChlorophyllMeasuredbytheOptic-fiber SurfacePlasmonResonanceSensor FENGWei-zhong,HANYan,YANGJing,ZHANGChong (CollegeofInformationScienceandTechnologyNanjingForestryUniversity,Nanjing210037,China) Abstract:Theworkingprincipleoftheoptic-fiberSurfacePlasmonResonance (SPR)sensor isbasedontheresonanceofthesurfaceplasmon.SPRsensorhasbeenappliedtomeasurethe refractiveindexofsurroundingmaterial.Byestablishingthecorrespondencerelationshipbe- tweenSPRsignalandthesurroundingmaterial,theinsideinformationinfluencedbyrefrac- tiveindexcanbestudied.Thebasicconcept,detectionsystemandmethodusedtomeasure thecontentofchlorophyllbyoptic-fiberSurfacePlasmonResonancesensorareintroduced. Therelationshipbetween theresonantwavelengthandthecontentofchlorophyllfound throughexperimentscanbeutilizedtotestthecontentofchlorophyllfastandaccuratelywith thehelpoftheSPWsensor. Keywords :Surfaceplasmonresonance;Resonantwavelength;Refractiveindex;Chlorophyll