基于微流控芯片的酶及其抑制剂的研究进展Advanceson-药学学报.PDF

694 药学学报 Acta Pharmaceutica Sinica 2010, 45 (6) : 694698 基于微流控芯片的酶及其抑制剂的研究进展 侯凤华, 叶剑清, 陈缵光, 成志毅* ( 中山大学药学院, 广东 广州 510006) 摘要: 近 20 年来, 随着微流控芯片加工技术的不断发展, 微流控分析已从一个概念发展为当前世界上最前 沿的科技领域之一, 微流控芯片上酶及其抑制剂的研究也取得重要进展。微流控技术用于酶及其抑制剂研究时 在减少试剂用量、缩短分析时间、自动化等方面提高了分析性能。本文综述了微流控芯片上酶及其抑制剂的研 究。 关键词: 酶; 酶抑制剂; 微流控芯片 中图分类号: Q523 文献标识码:A 文章编号: 05 13-4870 (2010) 06-0694-05 Advances on enzymes and enzyme inhibitors research based on microfluidic devices * HOU Feng-hua, YE Jian-qing, CHEN Zuan-guang, CHENG Zhi-yi (School of Pharmaceutical Science, Sun Yat-Sen University, Guangzhou 510006, China) Abstract: With the continuous development in microfluidic fabrication technology, microfluidic analysis has evolved from a concept to one of research frontiers in last twenty years. The research of enzymes and enzyme inhibitors based on microfluidic devices has also made great progress. Microfluidic technology improved greatly the analytical performance of the research of enzymes and enzyme inhibitors by reducing the consumption of reagents, decreasing the analysis time, and developing automation . This review focuses on the development and classification of enzymes and enzyme inhibitors research based on microfluidic devices. Key words: enzyme; enzyme inhibitor; microfluidic chip 现代合成化学、 合化学和生物科学的迅速发展 领域的发展和完善。 使得大量新化合物迅速涌现, 为新药筛选提供了大 微流控芯片 (microfluidic chip) 技术是一项将 量的候选化合物。针对特定的酶, 每一种对其活性具 生物和化学等领域中所涉及的样品制备、生物与化学 有抑制效果的化合物都可能在治疗与之相关的疾病 反应、分离、检测及细胞培养等基本操作单元