小鼠MSCs体外定向诱导分化神经及脂肪细胞的研究-CiteSeerX.PDF

2008,41(1):229-236 Scientia Agricultura Sinica 1 2 266109 750021 Differentiation of Mouse Bone Marrow Mesenchymal Stem Cells into Neurocytes and Adipocytes Induced in vitro 1 1 1,2 1 ZHAO Xing , BAI Xue-jin , FENG Ji-wu , DONG Ya-juan 1 2 ( Animal Embryo Engineering Center, Qingdao Agricultural University, Qingdao 266109, Shandong; Agricultural College of Ningxia University, Yinchuan 750021) Abstract: Objective To establish a system for isolation and culture of bone marrow mesenchymal stem cells (MSCs) from Kun-ming mouse in intro and to identify the characteristics of the cells, directional neuronal and adipocytic induction after culture expansion. MethodMSCs were isolated and cultivated from the bone marrow of Kun-ming mice by investigating their adherence-dependent growth characters. The morphological characters of MSCs were observed under inverted phase contrast microscope. Shape characteristics of MSCs were studied by AKP staining, Hoechst33258 staining, white Giemsa staining. MSCs were induced into neurone-like cells by β-ME, and stained with H.E after induction, then their characteristics were observed under inverted phase contrast microscope. The differentiation ratio analysis of differentiated neurocytes was detected by immunocyte-fluorescence of NF-200 and NSE. MSCs were induced into adipocytes by horse serum, the process of growth in vitro, was observed and the differentiation ratio analysis of differentiat