干燥前水洗对丹参活性成分的影响-天津中草药杂志社.PDF

中草药 Chinese Traditional and Herbal Drugs 第 46 卷 第 16 期 2015 年 8 月 • 2467 • 干燥前水洗对丹参活性成分的影响 尉广飞，李 翠，刘 谦，李 佳，张永清* 山东中医药大学，山东 济南 250355 摘 要：目的 研究干燥前水洗对丹参 Salvia miltiorrhiza 活性成分量的影响，为规范丹参产地加工方法提供依据。方法 将 丹参鲜药材采取不洗、冲洗、浸洗、搓洗等方法处理，采用 HPLC 法测定其活性成分量的变化，Diamonail C18 色谱柱（250 mm × 4.6 mm ，5 μm ），乙腈-0.026%磷酸溶液梯度洗脱，体积流量为 1.0 mL/min，检测波长为286 和 270 nm 。结果 水洗后，迷 迭香酸、丹酚酸 B 、隐丹参酮、丹参酮I 、丹参酮II 5 种活性成分量均有所降低，水洗力度越大，质量分数降低幅度越大， A 搓洗丹参中的活性成分量最低。结论 水洗使丹参活性成分遭受损失，应尽量采用冲洗方式，既要减少与水接触的时间，又 要避免药材间摩擦及人工搓揉。 关键词：丹参；产地加工；水洗；迷迭香酸；丹酚酸 B ；隐丹参酮；丹参酮I ；丹参酮IIA 中图分类号：R282.4 文献标志码：A 文章编号：0253 - 2670(2015)16 - 2467 - 04 DOI: 10.7501/j.issn.0253-2670.2015.16.022 Influence of washing before drying on active components of Salvia Miltiorrhizae Radix et Rhizoma WEI Guang-fei, LI Cui, LIU Qian, LI Jia, ZHANG Yong-qing Shandong University of Traditional Chinese Medicine, Ji’nan 250355, China Abstract: Objective To research the influence of different washing methods before drying on the contents of active components in Salvia Miltiorrhizae Radix et Rhizoma, and provide the basis for standard original processing methods of Salvia miltiorrhiza. Methods The fresh herbs of S. miltiorrhiza were processed with different methods including no washing, water flushing, immersion cleaning, and scrubbing in water. HPLC was applied to determine the contents of active components, with Diamonsil C18 column (250 mm × 4.6 mm, 5 μm), acetonitrile-0.026% phosphoric acid for gradient elution, flow rate of 1mL/min, and detection wave length of 286 and 270 nm. Results The contents of rosmarinic acid, salvianolic B, crypotanshinone, tanshinone I, and tanshinone IIA were all significantly decreased in S. miltiorrhiza processed with different washing methods. The contents of active components were reduced with the increasing of washing intensi