应用微阵列技术筛选PS1TP1基因转染细胞差异表达基因.PDF

应用微阵列技术筛选 PS1 TP1 基因转染细胞差异表达基因 纪 　冬 ,成 　军 ,郭 　江 ,刘 　妍 ,王 　琳 ,郭风劲 (解放军第 302 医院传染病研究所基因治疗研究中心 ,北京 100039) Screening of Genes Diff erentially Expressed in Hep G2 Cell s Transf ected with Gene 1 Transactivated by Hepatitis B Virus Pres1 with Microarray Assay J I Don g , C H EN G J un , GU O J iang ,L IU Yan ,WAN G Lin , GU O Fen gj in ( ) Gene T herap y R esearch Center , I ns t it ute of I nf ect ious D iseases , 302 H osp it al of PL A , B eij i ng 100039 , Chi na Abstract : To under st and t he differentially exp re ssed t ar get gene s in Hep G2 cell s t ran sf ect ed wit h P S1TP 1 p rot ein exp re ssion vector , we co mp ared t he diff erentially exp re ssed gene s bet ween t he ( ) ( ) hep atobla sto ma cell line Hep G2 t ran sf ect ed by p cDN A3 . 1 and p cDN A 3 . 1 P S1TP 1 , re sp ec ti vely wit h cDN A microar ray . The P S1TP 1 co din g DN A f ragment wa s amp lified wit h p olymer ( ) ( ) a se chain reaction PCR . The exp re ssive vector of p cDN A 3 . 1 P S1TP 1 wa s con st r uct ed by ( ) routine molecular biolo gical met ho ds. The Hep G2 cell s were t ran sf ect ed by p cDN A 3 . 1 an d ( ) p cDN A