小鼠慢性应激性抑郁症易感品系筛选及其易感机制初步研究.PDF

/index.php/gjph Global Journal of Public Health, 2016, Vol.3, No.2 Screening of mouse strain susceptive to chronic stress depression and preliminary research of its susceptive mechanism 1 2 Long-jin XU , Ke-zhou Wang 1Shandong Center for Disease Control and Prevention, Ji’nan Shandong, China 2Experimental animal center of Shandong province, Ji’nan Shandong, China Received: Jun 02, 2016 Accepted: Jun 12, 2016 Published: Sep 16, 2016 DOI: 10.14725/gjph.v3n2a1516 URL: /10.14725/gjph.v3n2a1516 This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Objective: To screen mouse strain that susceptive to chronic unpredicted mild stress (CUMS) and preliminarily research its susceptive mechanism. Methods: KM, ICR, BABL/c and C57BL/6 mice were used to establish CUMS depression model. Body weight, sucrose preference test (SPT), open field test (FT) were used to evaluate the sensitivity of mice to CUMS. Hippocampal levels of glucocorticoid receptor (GR), 5-hydroxytryptamine (5-HT), kynurenine (KYN) were measured and used for susceptive mechanism research. Results: Body weight, SPT, horizontal movement score and vertical movement score of C57BL/6 mice were found to susceptive to CUMS. SPT, horizontal movement score and vertical movement score of BABL/c showed similar tendency. Hippocampal levels of GR and KYN of normal C57BL/6 mice were significantly lower or higher than that of normal KM, ICR and BABL/c mice. Conclusions: Our study has led to the view that C57BL/6 mice are susceptive to CUMS and hippocampal levels of GR and KYN may be the main mechanism responsible for its susceptibility. Key word