棉花粉蚧化学感受蛋白基因的鉴定与进化分析-广东农业科学.PDF

东农业科学 2015 第 12 期 121 棉花粉蚧化学感受蛋白基因的鉴定与进化分析 赵 洁 陆永跃 华南农业大学昆虫学系袁 东 广州 510642冤 要 昆虫化学感受蛋白 chemosensory proteins 袁CSPs冤 泛存在于各种昆虫中袁与昆虫识别外界信息密 切有关遥 从棉花粉蚧 Phe nacocc us sole nops is T insley 冤转录组数据中分析尧获得 12 条化学感受蛋白基因袁分别命 名为 PsolCSP -PsolCSP 遥 这些基因与已报道的其他昆虫 CSPs 基因序列相似性较高袁与双翅 尧鳞翅 尧膜翅 1 12 及半翅 昆虫的化学感受蛋白相似性都在 10% ~50%之间袁说明CSPs 序列在物种之间高度保守遥研究结果为进 一步研究棉花粉蚧 CSPs 的分子结构和功能奠定了基础遥 关键词 棉花粉蚧曰 化学感受蛋白基因曰 进化 中图分类号 S435.622 文献标识码 A 文章编号 1004-874X 2015 冤12-0121-07 Identification and evolution analysis on chemosensory protein genes in Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae) ZHAO J ie, LU Yong-yue (Development of Entomology, South China A gric ultural Unive rsity, Guangzhou 510642, China) A bstract: Insect chemosensory proteins are small soluble proteins expressed in various insect species, w hich are believed to be involved in chemosensory communication. In this study , based on the successful transcriptome sequencing in Phe nacocc us sole nops is, we identified 12 putative chemosensory protein (CSPs) genes 袁namely PsolCSP -PsolCSP , respectively . W ith 10% -50% similarities to Lepidoptera, Hymenoptera and Hemiptera, these 1 12 genes had high homology w ith the reported CSPs in other insects, w hich demonstrated the highly conservative of CSPs different species. T he results are helpful for further research on molecular structure and function of CSPs in P. sole nops is. Key