淫羊藿苷的提取分离和抗肿瘤作用机制的研究进展.PDF

现代药物与临床 Drugs Clinic 第 26 卷 第 5 期 2011 年 9 月 • 353 • 淫羊藿苷的提取、分离和抗肿瘤作用机制的研究进展 1 1 2* 蒋凌云 ，刘光明 ，陈可欣 1.大理学院 药学与化学学院，云南 大理，671000 2.大理学院 基础医学院，云南 大理，671000 摘 要：综述了近年来淫羊藿苷的提取分离方法和抗肿瘤活性及其机制。目前报道的提取方法有醇提法、超声法、微波法、 高压法，分离方法有柱色谱法（聚酰胺法和大孔吸附树脂法）、聚酰胺色谱联用醋酸乙酯萃取法等。淫羊藿苷及其衍生物对 肺癌、胃癌、肝癌、乳腺癌、白血病细胞均有一定的抑制活性，主要作用机制为淫羊藿苷通过影响肿瘤细胞周期，下调与肿 瘤细胞有关的信号通路及细胞因子的表达，抑制肿瘤细胞的增殖与转移，最终使肿瘤细胞凋亡。 关键词: 淫羊藿苷；提取分离；抗肿瘤；肿瘤细胞增殖；抑制肿瘤细胞转移；细胞凋亡 中图分类号：R284.2 ；R285.5 文献标志码：A 文章编号：1674 - 5515(2011)05 - 0353- 06 Advances in study on icariin extraction, separation, and its anti-tumor mechanism 1 1 2 JIANG Ling-yun , LIU Guang-ming , CHEN Ke-xin 1. School of Pharmaceutical and Chemical, Dali University, Dali 671000, China 2. School of Basic Medicine, Dali University, Dali 671000, China Abstract: The methods for the extraction and separation of icariin, and the mechanism of anti-tumor activity of icariin are reviewed in this paper. There are various extraction and separation methods have been reported, such as ethanol extraction, ultrasonic, microwave, and high pressure; column chromatography (polyamide and macroporous resin adsorption methods), polyamide chromatography combined with ethyl acetate extraction and so on. Icariin and its derivatives show the potential inhibition on several tumor cells, such as lung cancer, gastric cancer, liver cancer, breast cancer, and leukemia cells. Previous study showed that icariin could induce the cell apoptosis through arresting the cell cycle, inhibiting the tumor cell signaling pathways, down-regulating cell factor,expression, and suppressing the tumor cell proliferation and metastasis. Keywords: icariin; extraction and separation; antitumor; tumor cell proliferation; tumor cell metastasis; apoptosis 淫羊藿来源于小檗科淫羊藿属植物淫羊藿 为黄酮类化合物，其