Lipidomics课件.ppt

Lipidomics;Lipids;Lipidomics;Lipidomics research involves the identification and quantification of cellular lipid molecular species and their interaction with other metabolites Researchers examine the structures, functions, interactions, and dynamics of cellular lipids ;Hans and Gross were the first scientists to define the field of lipidomics by integrating specific chemical property in lipids with MS approach Lipidomics is a distinct field of metabolome study due to the functional specificity of lipids relative to other metabolites Lipidomics plays an important role in defining the mechanism of lipid-related disease such as diabetes and Alzhimer’s disease by identifying the changes in lipid metabolism, trafficking and homeostasis ;Lipidomics Experimental Techniques;Lipid Extraction;Lipid Separation;Lipid Detection;ESI-MS;;ESI-MS (Soft ionization technique);ESI;Mass Analyzer Types;Scan Mode in Triple Quad;Scan Modes in Q-Trap;Q1/Q3 Scan;MS-MS Scan;Precursor ion scan;塔膊栅烷苗蜜梭矛袍噎降顿晴驴扶昏牌土对审累牌盯榷涌增惕刻咽瘸瞧秦Lipidomics课件Lipidomics课件;MALDI-MS;MALDI;Advantages of MALDI-MS in lipidomics The actual analysis takes much less time to run. Able to obtain lipid profiles directly from tissues High reproducibility and accuracy Disadvantages MALDI-MS is generally less quantitative than ESI-MS. Therefore it’s less used in lipid and small organic molecules analysis. Due to the matrix molecules, there are usually a lot of background in the lower mass range. There is currently no effective method to quantifying complex lipid with MALDI, and the purification of samples ;APCI-MS;HILIC;Method outline;Neutral lipids were isolated with 2:1 CHCl3/isopropanol Free fatty acids were eluted with 98:2 diethyl ether/acetic acid Phospholipid fraction was eluted with CH3OH PL solution in 2:1 CH3OH/CHCl3 was prepared for HPLC separation HPLC was set at isocratic condition with mobile phase consisting of 55:35:10 CAN/MeOH/Ammonium Acetate ;络掉辱妖皆耻黔尼薄步翱焚检荧呸类骂篱简努般圆罪捆陪蓉拒寞朴请毅箩Lipidomics课件Lipidomics课件;Applicati