3株环链拟青霉固体培养条件的研究.pdfVIP

安徽农业大学学报，2008，35(1)：38-41 Journal of Anhui Agricultural University 3株环链拟青霉固体培养条件的研究 朱新燕 ，李增智 ，樊美珍 ，鲍素敏 ，杨震元 (1．安徽农业大学微生物防治省重点实验室，合肥230036；2．安徽农业大学生命科学学院，合肥230036) 摘 要：通过测定茵落直径和产孢量，研究不同温度、培养基和光照时间对3株环链拟青霉的茵丝生长和产孢 量的影响。结果表明，在23℃时，3个菌株茵落的直径生长最快，菌株Pc45和Pe287的产孢最多，而菌株Pc305在 27℃下产孢最多。与常用的PDA和SDAY培养基相比，3个茵株在改良米饭一蛋白胨培养基上的茵落直径生长和 产孢量均显著高于其他培养基；Mg 和K 对产孢有重要影响。3个菌株均以光照24 h下的产孢量最高，而在0 h 下，茵落直径最大，产孢量最小。 关键词：环链拟青霉；培养基筛选；温度；产孢量；光照时间；茵落直径 中图分类号：$476．12 文献标识码：A 文章编号：1672—352X(2008)01-0038-04 Solid culture conditions of three strains of Paecilomyces catenlannulatus ZHU Xin．yan ，LI Zeng．zhi ，FAN Mei-zhen ，BAO Su．min ，YANG Zhen-yuan (1．Anhui Provincial Key Laboratory of Microbial Con~ol，Anhui Agricultural University，Hefei 230036； 2．School of Life Science，Anhui Agricultural University，Hefei 230036) Abstract：Three strains of Paecilomyces cateniannulatus were incubated on solid culture under different tem- peratures，media and illumination periods．The result showed that the optimum temperature for mycelial growth of all 3 strains and conidiation of Pc45 and Pc287 was at 23％，while optimum conidiation temperature for the strain Pc305 was at 27℃．As compared to commonly used PDA and SDAY for entomogenous Paecilomyces spp．．an im- proved rice-peptone medium resulted in mycelial growth and conidiation significantly higher than the other media． Mg and K exerted obvious effect on conidiation of the fungus．The colonial diameter under 0 h illumination and the conidiation under 24 h were highest on both improved rice-peptone medium and a synthetic potato medium． Key words：Paecilomyces cateniannulatus；medium screening；temperature；conidiation；light duration；colony diamete 在调查马尾松林昆虫病原真菌资源时常发现， 链拟青霉可以发展为真菌杀虫剂，Shimazu已证明， 环链拟青霉 (Paecilomyces c0 n 0肌u us)是松林 该制剂用于防治柳树害虫具有较高的防效 。另 生态系中仅次于球孢白僵菌的