5-羟色氨2A受体对新生大鼠延髓脑片节律性呼吸放电的调制作用.pdfVIP

新乡医学院学报 Journal ofXinxiangMedical College Vo1．25 No．1 Jan． 2008 ·2l· 5一羟色氨2A受体对新生大鼠延髓脑片节律性呼吸放电的调制作用 千智斌 ，姬明丽 ，吴中海 (1．南方医科大学生理学教研室，广东 广州 510515；2，新乡医学院基础医学院，河南 新乡 453003) 摘要： 目的 探讨5一羟色氨 (5一HT2 )受体对新生大鼠延髓脑片节律性呼吸放电的调制作用。方法 制作新生 大鼠离体延髓脑片标本，给予灌流恒温改良Kreb’S液，记录舌下神经根呼吸相关节律性放电活动(RRDA)。标本分2 组：第1组使用含5-HT2 受体特异激动剂2，5一二甲氧基4一碘苯基丙烷(DOI)的Kreb S液灌流脑片，观察其对RRDA的 影响；第2组使用含5一HT2 受体特异拮抗剂酮舍林的Kreb S液灌流脑片，观察其对RRDA的影响。结果 DOI延长 RRDA吸气放电时程(1I)、缩短呼气时程(TE)、缩短呼吸周期(RC)、增强RRDA放电积分幅度(IA)，对RRDA有兴奋 作用(P0．o1)。酮舍林缩短RRDA的1I，延长TE和RC、减弱IA，对RRDA有抑制作用(P0．o1)。结论 5-HT 受 体参与了新生大鼠基本呼吸节律的产生和调节。 关键词： 呼吸相关节律性放电；5一羟色氨 受体；延髓脑片 中图分类号：R338．2 文献标识码：A 文章编号：1004-7239(2008)01—0021—03 Modulation of 5-HT2A receptors on rhy thmic respiration discharge activity in the transverse medullary slice of neonatal rats QIAN Zhi—bin 一，JI Ming—li ，wU Zhong—hai (1，Department ofPhysiology，Southern Medical University，Guangzhou 510515，China；2．College ofBasic Medical Science， 砒一 iang Medical College，Xinxiang 453003，China) Abstract： Objective To study the modulation of 5-HT2A receptors Oil rhythmic respiration discharging activity in the transverse medullary slices of neonatal rats．Methods The transverse medullary slices of neonatal rats were made and perfused with homothermal and improved Kreb／s liquid，the respiratory—related rh~hmic discharge activity(RRDA)of hypoglossal nerve root was recorded．The slices were divided into two groups，In group I，the slices were perfused with 5-HT2A receptor excitomotory 1一(2，5-dimethoxy-4一iodopheny1)-2一aminopropane(DOI)dissolved in modified Kreb S solution．In group II，the slices were perfused with ketanserine specific antagonist of 5-HT2A receptor．The effects of DOI and ketanserine on RRDA were observed．Re- suits DOI could increase RRDA，prolonged inspiratory time(rIII)，shortened expiratory time(TE)and respiratory cycle (RC)，increased integral amplitude(IA)of RRDA(P0．01)．Ketanserina could inhibit RRDA，shortened TI，prolonged TE and RC，decrease