40株虫生真菌菌丝体提取物抗肿瘤活性的初步研究.pdfVIP

安徽农业大学学报，2008，35(1)：84—88 Journal of Anhui Agricultural University 40株虫生真菌菌丝体提取物抗肿瘤活性的初步研究 何亚琼，胡丰林 ，李春如，左登平，刘玉军，樊美珍 (安徽农业大学微生物防治省重点实验室，合肥230036) 摘 要：以中国仓鼠卵巢(CHO)细胞株为模型，用Resazurin法检测生长抑制率，对40株虫生真菌菌丝体的提 取物进行体外抗肿瘤活性试验。结果表明，黄山被毛孢HirsuteUa huangshanensis菌株RCEF0851和RCEF0868菌 丝体提取物对CHO细胞株的抑制活性最强，其乙酸乙酯和二氯甲烷混合溶剂提取物在100 tzg·mL 时对CHO细 胞的抑制率分别为(98．58 4-1．12)％、(98．47 4-2．11)％；其甲醇提取物在100 g·mL 浓度时对CHO细胞的抑制 率也分别达到(97．30 4-1．83)％和(96．28±1．96)％。复筛的结果表明，RCEF0851和RCEF0868菌丝体提取物对 CHO细胞的半数抑制浓度 ，C5o分别为29．24 g·mL 和28．87 g·mL～。另外，环链拟青霉 Paecilomyces cateniannulatus菌株RCEF0508菌丝的乙酸乙酯和二氯甲烷混合溶剂提取物在100 tzg·mL 时对CHO细胞的抑制 率也达(95，96 4-2．01)％，这些菌株值得进一步深入研究。 关键词：虫生真菌；抗肿瘤活性；CHO细胞 中图分类号：Q949．32；R282．71 文献标识码：A 文章编号：1672-352X(2008)01-0084-05 Antitumor activities of extracts from mycelium of 40 entomogenous fungi HE Ya—qiong，HU Feng—lin，LI Chun—ru，ZUO Deng—ping，LIU Yu-jun，FAN Mei—zhen (Anhui Provincial Key Laboratory of Microbial Control，Anhui Agricultural University，Hefei 230036) Abstract：A resazurin cytotoxity assay model with Chinese hamster ovary(CHO)cells was employed to study antitumor activity of extracts from mycelium of 40 entomogenous fungi strains．Pre—screening revealed that extracts from strains RCEF085 1 and RCEF0868 of Hirsutella n凡gs n凡e凡s have the strongest antitumor activity．The in— hibitory rates of the ethyl acetate and methylene chloride mixture(1：1)extracts were(98．58±1．12)％ and (98．47±2．1 1)％at the test concentration of 100 I．zg·mL～，respectively，and the inhibitory rates of the methanol extracts were(97．30±1．83)％ and(96．28±1．96)％ at the same test concentration．The secondary screening revealed that the IC5o of ethyl acetate and methylene chloride mixture(1：1)extracts from mycelium of strains RCEF085 1 and RCEF0868 were 29．24 Ixg‘mL～ and 28．87 I．zg‘mL～，respectively．Additionally，the et