黄瓜枯萎病菌毒力营养体亲和性及ISSR分析-植物病理学报-中国.PDF

植物病理学报 ACTA PHYTOPATHOLOGICA SINICA　 42(5):456-465(2012) 黄瓜枯萎病菌毒力、营养体亲和性及 ISSR分析 刘 东, 代丽婷, 蒲子婧, 苗 笛, 马柏壮, 张艳菊* (东北农业大学, 哈尔滨 150030) 摘要:本研究对来自哈尔滨、长春、沈阳、北京、西宁5个城市的70个尖孢镰刀菌黄瓜专化型菌株进行了毒力、营养体亲和 性及ISSR分析。 毒力测定结果显示黄瓜枯萎病菌在东农803 品种上存在明显的毒力分化。 在营养体亲和群的测定中有8 个菌株没有产生nit突变体,2个菌株经测定为异核体自身不亲和性菌株,不能进行营养体亲和群的测定;其余60个菌株可 分为5个营养体亲和群。 利用筛选的7个引物对70个菌株进行了ISSR分子标记,聚类分析可将70个菌株分为3个类群, 其中IG Ⅰ的41个菌株均来自东北三省,IGⅡ的21个菌株均来自北京,IGⅢ的8个菌株全部来自西宁。 VCGs和ISSRs与 菌株的地理来源及毒力存在一定的相关性。 关键词:黄瓜枯萎病菌;毒力分化;营养体亲和群;ISSR Virulence,vegetativecompatibility and ISSRanalysisof Fusariumoxysporumf.sp. cucumarinum　 LIU Dong, DAI Li-ting, PU Zi-jing, MIAO Di, MA Bai-zhuang, ZHANG Yan-ju (Northeast Agricultural University,Harbin 150030,China) Abstract: To study genetic diversity of Fusarium oxysporum f.sp. cucumarinum,70 isolates from Harbin, Changchun,Shenyang,Beijing and Xining were tested by virulence,vegetative compatibility and ISSR. The results showed that evident virulence differentiation of pathogenic isolateswaspresent in Dongnong803 culti- var. The test of vegetative compatibility of these isolatesindicated that8 isolates didn’t produce nit mutants, and2 isolateswere heterokaryon self-incompatibile and therefore could not preceed to the vegetative compati- bility analysis. Finally,five vegetative compatibility groups were identified among the remnant 60 isolates. The genetic diversity of the isolates was analyzed with 7 ISSR primers and resulted in three ISSR groups (IG). IG Ⅰincluded41 isolates from three provinces of Northeastern China. IGⅡincluded 21 isolates from Beijing,and IGⅢincluded 8 isolates from Xining. It suggested that VCGs and ISSRs were correlated with groups defined by geographical origin and virulence. Key words: Fusarium oxysporum f. sp. cucumarinum; virulence differentiation; vegetative compatibility groups;ISSR 中图分类号: S436.421.13　 　 　 　 　 文献标识码: A　 　 　 　 　 文章编号:0412-0914(2012)05-0456-10 　 　 黄瓜枯萎病