高效液相色谱法同时检测食品中的碱性橙碱性嫩黄o.pdf

194 2009, Vol. 30, No. 14 食品科学 ※分析检测 高效液相色谱法同时检测食品中的碱性橙、 碱性嫩黄O和碱性桃红T染料含量 林 钦，郑小严，何树坤，戴 明，谢 勇 (福建省中心检验所，福建福州 350002) 摘 要：建立了食品中碱性橙、碱性嫩黄O 和碱性桃红T 染料含量的高效液相色谱检测方法。样品经碱化甲醇提 取，提取液在碱性条件下用二氯甲烷萃取，浓缩后用酸化甲醇溶解并用甲醇饱和正己烷萃取净化后经高效液相色谱 分离、检测。本方法检测限分别为碱性橙0.02mg/k g 、碱性嫩黄O 0.03mg/k g 、碱性桃红T 0.004mg/kg 。6 种食品 样品加标回收率实验所得回收率为72 .3% ～96 .5% ，相对标准偏差(R SD)为0.3% ～8.8%(n=6) ，三种染料在0.05～ 2 .5 μg/ml 浓度范围内均呈良好的线性关系，线性回归系数(r)均大于0.9998 。 关键词：高效液相色谱法；食品；碱性橙；碱性嫩黄O ；碱性桃红T Simultaneous High Performance Liquid Chromatographic Determination of Chrysoidine, Auramine O and Safranine T in Food LIN Qin ，ZHEN G Xiao-yan ，HE Shu-kun ，DAI Min g ，XIE Yong (Fuj ian Provincial Central Inspection In stitute, Fuzhou 350002, China) Abstract ：A method for the simultaneous determination of chrysoidine, auramine O and safranine T in food was developed by high performance liquid chromatography (HPLC). Samples were extracted using ammonia－methanol (1:50, V/ V). Then the analytes were extracted from the extracts using CH2Cl2 , concentrated and redissolved in formic acid －methanol (1:50, V/ V), and cleaned up using n-hexane solution saturated with methanol prior to HPLC determination. The linear ranges of this method for determining the three pigments were 0.05－2.5 μg/ml and the correlation coefficients (r) all were higher than 0.9998. The recoveries for them in 6 kinds of foods spiked at two levels respectively ranged from 72.3% to 96.5%, and the RSDs were 0.3% －8.8% (n = 6). The limits of detection were about 0.02 mg/kg for chrysoidine, 0.03 mg/kg for auramine O and 0.004 mg/kg for safranine T. Key words ：high performance liquid chromatography ；food ；chry soidine ；auramine O ；safranine T 中图分类号：TS264.4 ；R 155