不同种源何首乌的ITS序列分析及其亲缘关系研究严寒静.pdf

, 2008, 2 8(5):0922-0927 Acta Bot.Boreal .-Occident .Sin. 　　:1000-4025 (200 8)05-0922-06 ＊ ITS 1, 2 2 1 ＊ 严寒静 ,房志坚,余世孝 (1 , 5 10275;2 , 5 10006) 　:P CR , 10 DN A IT S , Ge nBank IT S (Polygonum aviculare ), 。:(1)I TS 1 195 bp, G+C 69.23%～ 72.31%, IT S2 189 bp, G+C 77.25%～80.95 %, 0.001 75 ～0.049 45;(2)10 2 , , ;(3)、, ———。 :;IT S ; :Q 789 :A ITS Sequences and Phylogenetic Relationships of Fall op ia mul tif lora from Different AreasBased on Evidences 1,2 2 1 ＊ YA N H an-j ng ,F A NG Zh -j an , Y U Sh -x ao (1 School of L fe S c ence, S un Yat-sen U n vers ty, Guangzh ou 510275, Ch na;2 School of Ch nese T rad t on M ed cal, Guangdong U n vers ty of Pharmacy, Guangzhou 5 10006, Ch na) Abstract:In order t o resolve the relat o nsh p s am ong Fallop ia multif lora populat ons, IT S (nternal tran- scr bed spacer )sequences o f 10 populat ons w ere u sed t o analy ze the r sequence d ve rg ence and t o const ruct phylo genet c t ree n th s paper .Polygonum aviculare w ere u sed as the outg roup.So me conclus o ns could be ach eved as fo llow s (1)the sequence of I TS 1 n 10 populat ons w as 195 bp n lengt h, and t he G +C content var ed f rom 69 .23%to 72.31%.T he sequence of IT S2 w as 189 bp.T he G +C co nt ent v ar ed f ro m77.25% to 80 .95%.T he genet c d stances v ar ed f rom 0.00 1 75 t o 0.049 45;(2)T en popula t ons of F.multif lora can be class f ed as t w o clades naturally acco rd ng t o the r clu ste r dendro gram .T he populat on f rom T an- y ang w as an ndependent branch, so t he k nsh p be tw een T any an and o thers w ere fa r.T he o ther po pula- t ons w ere clu stered to get her ;(3 )T