栀子大黄汤血清药物化学的初步研究唐峥.pdf

中草药 Chinese Traditional and Herbal Drugs 第 45 卷 第 23 期 2014 年 12 月 ·3377 · 栀子大黄汤血清药物化学的初步研究 1 2* 2 2 2 2 唐 峥 ，尹 然 ，毕开顺 ，朱鹤云 ，张晓书 ，毛新娟 1. 沈阳药科大学中药学院，辽宁 沈阳 110016 2. 沈阳药科大学药学院，辽宁 沈阳 110016 摘 要：目的 对栀子大黄汤（Zhizi Dahuang Decoction ，ZDD ）的入血成分进行分析研究，进而探讨其药效物质基础。方 法 采用血清药物化学研究方法，建立口服 ZDD 后大鼠血清的超快速液相色谱（UFLC ）指纹图谱；通过比较ZDD 、缺味 方、单味药以及各组给药后所得血清样品，初步确定口服 ZDD 后大鼠血中移行成分及其来源生药。结果 口服 ZDD 后在 大鼠血中发现了 21 个入血成分，其中 12 个为代谢产物，9 个为原型成分；经与对照品比对，指认了 3 个原型入血成分，分 别为京尼平苷、芦荟大黄素和大黄酸。结论 初步确定了 ZDD 的入血成分，为其药效物质基础的阐明奠定了基础。 关键词：栀子大黄汤；血清药物化学；入血成分；指纹图谱；物质基础；京尼平苷；芦荟大黄素；大黄酸 中图分类号：R286.02 文献标志码：A 文章编号：0253 - 2670(2014)23 - 3377 - 06 DOI: 10.7501/j.issn.0253-2670.2014.23.004 Preliminary study on serum pharmacochemistry of Zhizi Dahuang Decoction 1 2 2 2 2 2 TANG Zheng , YIN Ran , BI Kai-shun , ZHU He-yun , ZHANG Xiao-shu , MAO Xin-juan 1. School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China 2. School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China Abstract: Objective To study the blood components in Zhizi Dahuang Decoction (ZDD) and to further explore its pharmacodynamic material basis. Methods UFLC fingerprints of serum in rats after ZDD treatment were established with serum pharmacochemistry method. The UFLC profiles of serum samples collected after the treatment of ZDD, ZDD without one of the component herbs and single crude herb were compared, and the transitional constituents to blood and their metabolites were analyzed. Results Twenty-one constituents absorbed into rat blood were detected after oral administration of ZDD, 12 of which were metabolites. Other nine were prototype constituents, three of which were recognized as geniposide, aloeemodi