2011.02.20 黄早四抗玉米丝黑穗病近等基因系的创建_邢跃先.pdf

, JounalofAnhuiAg.iSc.i20 11, 39(6 ):3368-3370 　高晓余　　傅真治 1 1 1 1 1 2 2 1＊ 邢跃先 ,吴凤新 ,蔡鑫茹 ,孙志超 ,夏远峰 ,晁青 ,徐明良 ,檀国庆 　 (1., 136100;2., 100094) 　[目的]创建黄早四抗 米丝黑穗病近等基因系, 为其在育种和生产中的广泛应用奠定基础。 [方法] 采用常规的杂交、回交、自 交技术与分子标记辅助选择相结合, 对黄早四进行了抗丝黑穗病的成功转育, 并对其近等基因系进行研究,观察回交转育成的近等基因 系的抗感变异及配合力变化特点。 [结果]对照黄早四发病率 46%, 入选的 25 个抗病黄早四近等基因系发病率均小于 10%, 入选率为 92.6%;多数抗病黄早四近等基因系与对照黄早四之间的配合力差异不显著, 而M135、M140等几 个自交系所配制的杂交种产量突出, 超 过对照郑单 958, 表现出较高的配合力。 [结论]试验所得抗 米丝黒穗病黄早四近等基因系的表型和配合力都已经非常接近黄早四, 而其抗 米丝黑穗病能力大大提高, 从而解决了黄早四不抗 米丝黑穗病的难题。 　黄早四; 米丝黑穗病;近等基因系;分子标记 + 　S435.131.4 2　　　A　　　0517-6611(2011)06-03368-03 EstablishmentofNeaIsogenicLinesofHuangzaosiMaizewithResistanceagainstMaizeHeadSmut XINGYue-xianeta　l(JilinAcademyofAgicultualScience,sGongzhuling, Jilin136100 ) Abstact　[Objective] ThepapewastoestablishtheneaisogeniclinesofHuangzaosimaizewithesistanceagainstmaizeheadsmu,tandto povidebasisfoitswideapplicationinbeedingandpoduction.[Method] Combingwithhybid, backcossandself-cossmethod, aswellasmo- leculamake,sHuangzaosimaizewassuccessfullytansfeedwithheadsmutesistanc,eanditsneaisogeniclinesweestudied.Thechaacteis- ticsofesistantandsusceptiblevaiationandthechangesofcombiningabilityofneaisogeniclinesaftebackcossingtansfeweeobseved. [Resul]tTheincidenceateofHuangzaosimaizeincontolwas46%;theincidenceatesoftheselected24 neaisogeniclinesofesistantHuang- zaosiweealllowethan10%, andtheselectedatewas92.6%;thecombiningabilityofmostneaisogeniclinesofesistantHuangzaosimaize hadnosignificantdiffeencewiththatofHuangzaosimaizeinconto;ltheyieldsofhybidspepaedbysevealinbedlinessuchasM135 and M140 weeoutstanding, whichexceededthecontolZhengdan958, showingahighecombiningability.[Conclusion] Thephenotypeandcomb-i ningabilityofneaisogeniclinesofHuangzaosimaizewithesistanceagainstmaizeheadsmutobtainedinthetestweeveyclosetoHuangzaos,i anditsesistanceagainstmaizeheadsmutwasgeatlyinceased,