hbv感染所致慢加急性肝功能衰竭患者hbv变异的纵向研究.pdf

DOI :10.14000/j .cnki .i ssn.1008 -1704.2010.03.038 2010 6 15 3 ·155 · ·论　著 · HBV HBV 闫涛　李克　李梵　王慧芬 　　【】　　(HBV )(A CLF)。　 , 6 HBV , ACLF 4 (P 1～P4), (LC)2 (P5 、P6 )。 2 , HBV DNA 、、, Vector NT I Suite 9.0 , H BV 。　6 C 。A CLF (P1 ～P4) HBV (P 5、P6)。A CLF , nt53 (C※T T※C)、nt184 6(A※T )、nt189 6(G※A ) 2 2 。C1913 A T P1、P2, P2 。　nt53、nt1846 、 nt189 6 nt1913 A CLF 。 【】　;; Longitudinal study of HBV mutations in patients with acute on chronic liver failure　　　　YAN Tao, L I K e, LI Fan, WAN G H ui-f en.I ntensive Care Center of 3 02 Military Hosp ital, Beij ing 100 039, China Corresp on ing author:WA N G Hui-f en, E-mail:wanghuif en@me 【Abstract】　Objective 　T o clarify whether H BV mutations are associated with acute on chronic li er failure (A CLF ).Methods　　Longitudinal study w as employ ed.Serum samples from the pre-AC LF stage, as w ell as during the occurrence of A CLF were obtained from 4 patients (P1 ～ P4 );Another 2 serum samples w ere collected f rom chronic hepatitis stage and li er cirrhosis stage (P5, P6 )as cont rol.HBV DNA w as extracted, amplified and ligated into modified PCR 2 .1 plasmid and finally sequenced.The results of the sequences were aligned betw een pre-A CLF and post- ACLF and /or pre-cirrhosis and post cirrhosis.Results　All patients were infected w ith HBV genotype C.There w ere more longitudinal nucleotide mutations in patients with A CLF than those with LC alone.Longitudinal mutations presented in more than 1 A CLF patients w ere nt53, nt1846 and nt1896.And nt19 13 mutation in the course of disease w as presented in patient P2, and C1913 A or G mutation w as seen in P1 and P2 at A CLF stage.Conclusion　Mutation at n