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论著闫涛李克李梵王慧芬
DOI :10.14000/j .cnki .i ssn.1008 -1704.2010.03.038
2010 6 15 3 ·155 ·
·论 著 ·
HBV
HBV
闫涛 李克 李梵 王慧芬
【】 (HBV )(A CLF)。
, 6 HBV , ACLF 4 (P 1~P4), (LC)2 (P5 、P6 )。
2 , HBV DNA 、、, Vector NT I Suite 9.0 ,
H BV 。 6 C 。A CLF (P1 ~P4) HBV
(P 5、P6)。A CLF , nt53 (C※T T※C)、nt184 6(A※T )、nt189 6(G※A )
2 2 。C1913 A T P1、P2, P2 。 nt53、nt1846 、
nt189 6 nt1913 A CLF 。
【】 ;;
Longitudinal study of HBV mutations in patients with acute on chronic liver failure YAN Tao, L I K e, LI Fan,
WAN G H ui-f en.I ntensive Care Center of 3 02 Military Hosp ital, Beij ing 100 039, China
Corresp on ing author:WA N G Hui-f en, E-mail:wanghuif en@me
【Abstract】 Objective T o clarify whether H BV mutations are associated with acute on chronic li er failure
(A CLF ).Methods Longitudinal study w as employ ed.Serum samples from the pre-AC LF stage, as w ell as during the
occurrence of A CLF were obtained from 4 patients (P1 ~ P4 );Another 2 serum samples w ere collected f rom chronic
hepatitis stage and li er cirrhosis stage (P5, P6 )as cont rol.HBV DNA w as extracted, amplified and ligated into
modified PCR 2 .1 plasmid and finally sequenced.The results of the sequences were aligned betw een pre-A CLF and post-
ACLF and /or pre-cirrhosis and post cirrhosis.Results All patients were infected w ith HBV genotype C.There w ere
more longitudinal nucleotide mutations in patients with A CLF than those with LC alone.Longitudinal mutations
presented in more than 1 A CLF patients w ere nt53, nt1846 and nt1896.And nt19 13 mutation in the course of disease w as
presented in patient P2, and C1913 A or G mutation w as seen in P1 and P2 at A CLF stage.Conclusion Mutation at
n
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