齐墩果酸对蛛网膜下腔出血大鼠早期脑损伤的保护作用.pdf

·762 · 现代药物与临床 Drugs Clinic 第 32 卷 第 5 期 2017 年 5 月 齐墩果酸对蛛网膜下腔出血大鼠早期脑损伤的保护作用 * 韩雨薇，刘秀娟，高 阳，李晓明 沈阳军区总医院 神经外科，辽宁 沈阳 10016 摘 要：目的 探讨齐墩果酸对大鼠蛛网膜下腔出血后早期脑损伤的保护作用，阐释其作用机制。方法 72 只大鼠随机分 为假手术组、模型组和齐墩果酸 10、20 mg/kg 组，每组 18 只。采用颈内动脉穿刺法建立大鼠蛛网膜下腔出血模型，并在造 模 1 h 后给药，24 h 后进行神经功能损伤评分；检测脑水含率、脑伊文思蓝浸出；并利用 Western blotting 法检测脑组织 VE-cadherin 、p120-catenin 蛋白的表达量。结果 齐墩果酸（20 mg/kg ）可有效改善神经功能损伤（P ＜0.01 ）；降低脑水肿 和脑血管通透性（P ＜0.01 ）；增加VE-cadherin 、p120-catenin 蛋白的表达（P ＜0.01 ）。结论 齐墩果酸对大鼠蛛网膜下腔出 血后早期脑损伤具有显著的保护作用，改善脑水肿，其作用机制可能与增加脑血管内皮粘附连接蛋白的表达有关。 关键词：齐墩果酸；蛛网膜下腔出血；神经功能损伤评分；脑水肿；VE-cadherin 蛋白；粘附连接 中图分类号：R965 文献标志码：A 文章编号：1674 - 5515(2017)05 - 0762 - 05 DOI: 10.7501/j.issn.1674-5515.2017.05.002 Protection of oleanolic acid on early brain injury after subarachnoid hemorrhage in rat HAN Yu-wei, LIU Xiu-juan, GAO Yang, LI Xiao-ming Department of Neurology, General Hospital of Shenyang Military Command, Shenyang10016, China Abstract: Objective To explore the effect of oleanolic acid on early brain injury after subarachnoid hemorrhage and explain its mechanism. Methods SD rats (72) were divided into 4 groups: Sham, model, and oleanolic acid groups (10 and 20 mg/kg), and each group had18 rats. The endovascular puncture model was used to induce subarachnoid hemorrhage. Rats were ig administered with the according drugs after 1 h of model established. Neurological score, brain water content and blood-brain barrier were evaluated after 24 h. The protein expression of VE-cadherin and p120-catenin were determined by Western blotting. Results Oleanolic acid (20 mg/kg) could significantly improve neurological scores (P 0.01), reduced brain water content and blood-brain barrier permeability (P 0.01), and increased the levels of VE-cadherin and p120-catenin (P 0.01). Conclusion Oleanolic