血糖波动对糖尿病大鼠海马p38mapk表达及细胞凋亡蛋白baxbcl的.doc

研究报告 糖尿病血糖波动模型致大鼠海马体的炎性损伤 王 辉1，蔡月琴1，李守业2，徐志伟1，刘 琼1，寿旗扬1，李昌煜 （浙江中医药大学，杭州 310053，杭州 310053）链脲佐菌素treptozotocin，STZ）和葡萄糖制备SD大鼠糖尿病模型（M组）和持续高血糖模型（MS组），并错时腹腔注射给予葡萄糖、胰岛素制备糖尿病血糖波动大鼠模型（MF组）。血糖波动造模第6周时，测定大鼠一般生理学指标，血糖(lucose，Glu)、甘油三酯（riglyceride，TG）、低密度脂蛋白（ow density lipoprotein，LDL-C）和高密度脂蛋白（igh density lipoprotein，HDL-C）等血液生化指标；同时采用荧光定量PCR法检测大鼠海马体中IL-1β、IL-2、IL-6、IL-8、TNF-α等炎性因子mRNA的表达，Morris水迷宫试验检测血糖波动对糖尿病大鼠学习和空间记忆功能的影响。结果 （1）血糖波动造模第6周时，M组、MS组组（P0.01组组（0.01）。（2）尾静脉注射STZ 1周后，M组、MS组、MF组的Glu、TG、LDL-C都有显著性的提高（P0.01），HDL-C显著性下降（P0.01）。（3）与正常组比较，各模型组海马组织的IL-1β、IL-6、IL-8以及TNF-α均呈现显著性变化（所有P0.05），组间IL-2水平则差异无显著性（P0.05）。其中，血糖波动模型MF组IL-1β和TNF-α水平的变化最大，达到极显著水平（P0.01），并显著高于M组和MS组水平（P0.01）。尽管IL-2水平在各组间无统计学上的差异显著，仍可见血糖波动模型MF组中表达水平最低。（4）M组、MS组、MF组的逃避潜伏期经过平台的次数在平台象限内的游泳距离N组显著提高（P0.01）（P0.01）平台象限内的游泳距离（P0.05）【中图分类号】R33【文献标识码】A【文章编号】1671-7856（201）-0000-00 Doi:10.3969/j.issn.1671-7856.2013.004.000 Blood-glucose fluctuation induces inflammatory hippocampus damages in rat models of diabetes mellitus WANG Hui, CAI Yue-qin, LI Shou-ye, XU Zhi-wei, LIU Qiong, SHOU Qi-yang, LI Chang-yu （Zhejiang Chinese Medical UniversityHangzhou 310053, China; Zhejiang Medical College, Hangzhou 310053, China） Abstract】 Objective To analyze the effect of blood-glucose fluctuation on inflammatory hippocampus damage in rat models of diabetes mellitus. Methods Sixty male Sprague-Dawley rats in the age of 6-8 weeks and body weight of 180-210 g were included in this study. The diabetic rat model (M) was established by injection of streptozotocin (STZ). The persistent high glucose diabetic rat model group (MS) was established by intraperitoneal injection of glucose, and the blood-glucose fluctuation rat model group (MF) was developed by alternative intraperitoneal injection of insulin and glucose administration. Body weight, serum glucose (Glu), triglyceride (TG), low density lipoprotein (LDL-C）and high density lipoprotein (HDL-C) were measured at 6 weeks estab