人elisa检测试剂盒说明书.docx

oligomeric elisa试剂盒说明书，人elisa检测试剂盒oligomeric elisa试剂盒说明书，人elisa检测试剂盒产品规格：96T/48T。用途：科研实验等领域科学研究，不得用于临床诊断。Elisa试剂盒说明书，elisa试剂盒价格，ELISA检测试剂盒oligomeric elisa试剂盒说明书，人elisa检测试剂盒Kit operation procedure:Temperature and education: 30 minutes after the closure of the plate with a sealing plate, 37.With liquid: 30 (48T of 20 times) times the concentration of washing liquid with distilled water 30 (20 times the 48T) diluted after the backup.Washing: be careful torn off the seal plate membrane, discard liquid, drying, washing liquid to fill each hole, standing for 30 seconds after the discard, repeat 5 times, pat dry.Add enzyme: each hole is added with the enzyme labeled reagent 50 mu L, except the blank hole.Sample collection and storageSerum - using a serum separating tube to make the sample at room temperature or clot for two hours overnightCentrifugation of freshly prepared serum was measured at approximately 1000 x G. 4oC for 20 minImmediately or store the samples at -20 degrees C or diluted with 80oC. Avoid repeated freezing and thawing cycles.Plasma plasma collection with EDTA or heparin as an anticoagulant. 15 minutes, centrifugal samplesX G in 2 to 8 degrees Celsius for 30 minutes. Removal of plasma and immediate detection or storage of samplesLiquid type specimen:Including serum, plasma, urine, pleural effusion and ascites, cerebrospinal fluid, cell culture supernatant, etc..Serum:Room temperature blood natural coagulation 10-20 minutes after the centrifuge about 20 minutes (2000-3000 turn / min). Carefully collect the supernatant. In the process of preservation, if there is precipitation, should be re - centrifugal.Plasma):According to the requirements of the specimens of choice of EDTA, sodium citrate or heparin as an anticoagulant, 10-20 minutes after mixing, centrifuged for 20 minutes or so (2000-3000 r.p.m.). Carefully collect the supernatant. In the process of preservation, if there is precipitation, should be re - centrifugal.Urine:Collecting with sterile tubes