薄层层析txt电子书（Thin layer chromatography TXT e-book）.doc

薄层层析txt电子书（Thin layer chromatography TXT e-book） Also called thin layer chromatography TLC, a chromatographic method, rapid separation and qualitative analysis of a small amount of material is an important experimental technique is solid liquid adsorption chromatography, it has the advantages of column chromatography and paper chromatography, on the one hand, suitable for small samples (a few to a few grams, even 0.01 g) separation; on the other hand in the production of thin plates, the adsorption layer thickness increase, therefore, can be used to purify the sample, this method is especially suitable for the less volatile or high temperature and not easy to change with gas chromatography mass. In addition, thin-layer chromatography can also be used to track organic reactions and to pre test prior to column chromatography. Thin layer chromatography (Thin Layer Chromatography) commonly used TLC representation, also known as thin layer chromatography, belongs to solid liquid adsorption chromatography. It is a micro, rapid and simple chromatography developed in recent years. It has the advantages of column chromatography and paper chromatography. On the one hand, suitable for small samples (a few micrograms, or 0.01 g) separation; on the other hand if in the production of thin plates, the adsorption layer thickness, the sample points in a line, you can separate the sample up to 500mg. Therefore, it can be used to refine samples. Therefore, the method is especially applicable to materials which are less volatile or prone to change at higher temperatures and cannot be analyzed by gas chromatography. In addition, in chemical reactions, it is often used to observe the gradual disappearance of the spots of the material by thin-layer chromatography to determine whether the reaction has been completed. TLC is on the glass plate was washed clean (10 x 3cm) evenly coated with a layer of adsorbent or supporting agent with drying and activation of capillary droplet on sample sol