SDBC营养价值评定.doc

喷雾干燥破膜血球蛋白粉在断奶仔猪上的营养价值评定 邓莹莹 [摘要] 试验选用体重15.6±1.98kg DLY 仔猪12头，在测定血球蛋白粉常规养分和氨基酸含量基础上进行消化代谢试验。采用单因子试验设计，用无氮日粮半纯合日粮法测定蛋白粉猪回肠末端氨基酸的表观消化率MJ/kg，总氨基酸71.85%，其中主要限制性氨基酸：赖氨酸、蛋氨酸、苏氨酸、和异亮氨酸含量分别为6.32，0.91，2.30，0.36%。粗蛋白表观和真消化率分别为92.69%和94.34%，总氨基酸回肠表观消化率80.83%，消化能19.28MJ/kg，代谢能19.03MJ/kg。由此可见，血球蛋白粉的能量和蛋白质氨基酸营养价值 [关键词] 血球蛋白粉 营养价值 氨基酸消化率 仔猪 Nutritional Value of Spray-dried Animal Blood Cells for Weaned Piglets [Abstract] A digestibility trial to evaluate the nutritional value of spray-dried animal blood cells(SDBC) was conducted on the basis of analyzing the common composition and amino acid content. Twelve 15.6±1.98kg three-way crossbred DLY piglets were used in the experiment to determine the digestibility of crude protein, energy and amino acids of SDBC. N-free diet and semi-purified diet with SDBC as only protein source were used in two treatments each with six replications. The results showed that: SDBC includes crude protein 91.87%, GE 20.96 MJ/kg, general amino acids 71.85%, some of its main limited amino acid are: Lys 6.32%, Met 0.91%, Thr 2.30%,Ile 0.36%. The apparent and true digestibility of the crude protein were 92.69% and 94.34%, the apparent ileal digestibility of amino acids was 80.83%,the digestible energy and metabolic energy were 19.28MJ/kg and 19.03MJ/kg. It is concluded that SDBC has high nutritional value in terms of energy, protein and amino acids. [Key words] Spray-dried animal blood cells; nutritional value; amino acids digestibility；piglet 在我国饲料原料结构中，蛋白质饲料紧缺，特别是动物性蛋白饲料紧缺尤为严重，但许多饲料生产厂家习惯于用动物性蛋白饲料和植物性蛋白饲料配合来提供饲料成品的蛋白质，由于鱼粉、大豆资源日趋减少，价格一直高居不下，因此寻找廉价、优质且供应稳定的替代蛋白源非常重要。喷雾干燥破膜血球蛋白粉（Spray-dried animal blood cells，SDBC,使血细胞破坏,制备出变性血红蛋白,再经离心分离,沉淀物经浓缩及喷雾干燥制成的粉末。 1.2 SDBC营养成分分析 1.2.1 常规成分测定 参照杨胜主编(1993)《饲料分析及饲料质量检测技术》中的常规方法进行。蛋白质测定：微量凯氏定氮法；水分测定：常压干燥法；脂肪测定：索氏抽提法；灰分测定：灼烧重量法；钙测定：高锰酸钾滴定法；磷测定：钒黄法;能量的测定:用GR-3500型氧弹式热量计。 1.2.2 氨基酸含量的测定 采用L-8800全自动氨基酸分析仪，样品处理是用6mo1/L HC1, 110℃水解22h。 1.2.3 蛋白质溶解度（PS）的测定 参照杨胜主编(1993)《饲料分析及饲料质量检测技术》中测定大豆粕