三种植物源杀虫活性成分对东方粘虫中肠细胞的毒力测定-应用昆虫学报.PDF

　 ·676 · 　Chin s Bull tin of Entomology 2007 44(5) ＊ 1 2 1＊＊ 刘亚强 　彭大勇 　祁志军 (1.　　712100;2.　　330045) Evaluation of cytotoxicity of three botanical insecticidal activities against the midgut cell of Mythimna sep arata . 1 2 1＊＊ LIU Ya-Qiang , PENG Da-Yong , QI Zhi-Jun (1.Institute of Pesticide Science, Northwest Sci-Tech University of Agriculture and Forestry, Shaanxi 　712100, China;2.Collage of Science, Jiangxi Agricultual University, Nanchang Jiangxi　330045, China) Abstract　Th toxiciti s of c langulin V, fraxin llon and dictamnin against th midgut c ll of Mythimna se arata hav b n assay d with th m thods of MTT, N utral r d and Trypan blu stain, r sp ctiv ly.Th r sult show d that th midgut c ll gr w w ll in Grac s ins ct c ll cultur m dium.Th s thr phytoch micals hav obvious toxicity to th c ll.Th LC of c langulinⅤ against th midgut c ll by th m thods of MTT, N utral r d and Trypan blu stain 50 w r 9.08, 7.79 and 10.94 μg mL, thos of fraxin llon w r 27.85, 31.77 and 36.42 μg mL, and thos of th dictamnin w r 186.66, 164.00 and 192.34 μg mL. Key words　botanical ins cticidal activiti s, Mythimna se arata, midgut c ll, cytotoxicity 　　MTT([3-(4, 5--2)-2, 5-] )、 , Ⅴ、 。 :Grac s ;3 。MTT 、ⅤLC50 9.0, 7.79 10.94 μg mL;27.85, 31.77 36.42 μg mL; 186.66, 164.00 192.34 μg mL。 　, , , [3] 　　, 。 , , MTT 、 , , 3 、, Ⅴ、 , , , [ 1] 。, 。 , 1　 。 1.1　 Ⅴ :Grac s (gibicol ) , [2] ＊。 ;