南方红豆杉水提物对人肺癌A549细胞增殖抑制-浙江中医药学会.DOC

南方红豆杉水提物对人肺癌A549细胞增殖/抑制作用的研究 舒琦瑾1﹡1 朱国猛1 （1浙江中医药大学附属第一临床医学院肿瘤科，杭州310006；2杭州师范大学附属医院肿瘤科，杭州310015） 摘要：目的 研究南方红豆杉水提物对人肺癌A549细胞增殖的抑制作用和机制。方法 采用MTT法检测南方红豆杉水提物对A549细胞增殖的抑制作用；生长曲线法观察其抑制A549细胞的时间-效应曲线；流式细胞仪检测其对A549细胞周期及凋亡的影响；倒置显微镜及电镜观察其对A549细胞形态的影响。结果 南方红豆杉水提物对A549细胞的增殖有抑制作用；其抑制作用机制为使A549细胞周期出现明显的G0/G1期阻滞，诱导细胞凋亡。结论 南方红豆杉水提物对人肺癌A549细胞的增殖有抑制作用。 主题词：南方红豆杉；抑制作用；人肺癌A549细胞；细胞增殖 Study on Inhibitory Action of Proliferation of human lung carcinoma A549 Cell with aqueous extract of Taxus chinensis Shu Qi-jin1﹡, Li Ping2, Wang Bin-bin1, Zhu Guo-meng1 1.Department of Oncology,the First Affiliated Hospital,Zhejiang University of Traditional Chinese Medicine, Hangzhou 310006, China 2. Department of Oncology,the Affiliated Hospital of Hangzhou Normol University Hangzhou 310015, China Corresonding author: Shu Qi-jin, E-mail: shuqjhz@. Abstract：Objective To study the inhibitory effect of aqueous extract of T.chinensis var on vitro cultured human lung carcinoma A549 cell, and to explore its mechanism.Methods The inhibitory effect of T.chinensis var on human lung carcinoma A549 cell was tested by MTT method; time-effect relationship was observed by growth curve;effects on blockage of cell cycle and apoptosis was inspected by flow cytometry; morphology of the A549 cell was observed by inversion microscope and electron microscope. Results The aqueous extract of T.chinensis var has inhibitory effect on the proliferation of cell A549, and the mechanism related to blocking the cycle of cell A549 at G0/G1 period and inducing apoptosis of tumor cell. Conclusions The aqueous extract of T.chinensis var has inhibitory effect on the proliferation of cell A549. Subject words：T.chinensis var; inhibitory effect; human lung carcinoma A549 cell 红豆杉是红豆杉科红豆杉属植物总称，是世界上公认的濒临灭绝的天然珍稀抗癌植物。 《本草纲目》就记有红豆杉治疗霍乱、伤寒、排毒等疗效，《现代中药大辞典》、《本草推陈》等医药书中则有进一步记载。其性味苦、平，有小毒，归心经，功效消肿散结，主治：肿瘤、肾病、风湿等。目前已经从红豆杉中分离出400多种化合物，包括紫杉烷类化合物和少量的非紫杉烷类化合物[1-6]。 水煎剂是中药应用的传统方式及临床应用的主要方式，红豆杉水煎剂已在临床广泛应用,且安全有效。目前国内外尚未见应用红豆杉水提物抑制肿瘤的研究报