rf-pecvd聚合类聚乙烯氧peo-like薄膜的蛋白质吸附性研究.doc

RF-PECVD聚合类聚乙烯氧（PEO-like）薄膜的蛋白质吸附性研究 胡文娟1，谢芬艳1，付亚波1，陈强1*, 翁静2 1-北京印刷学院，北京大兴，102600）Ethylene Glycol DiMethyl Ether）为聚合单体,氩气为辅助气体,合成类聚乙烯氧（PEO-like）功能聚合薄膜。采用连续与脉冲射频（RF）等离子体两种放电模式聚合PEO功能薄膜。实验研究了等离子体放电参数：如等离子体放电功率、工作气压、放电模式（连续或脉冲）、聚合时间等对聚合物表面结构、功能团含量表面成分以及性能和蛋白质吸附等的影响。利用接触角测定仪（WCA）、傅立叶变换红外光谱(FTIR)、原子力显微镜（AFM）等手段对其进行结构、成分和形貌分析。同时本文也采用了体外细胞培养的方法研究了类PEO功能薄膜对富血血小板以及蛋白细胞的吸附。通过倒置显微镜观察细胞黏附的数量和形态。结论是：采用RF-PECVD可以在连续等离子体模式下较小的功率，或脉冲等离子体模式下较长时间得到结构、性能优异的PEO生物功能薄膜，所制备的PEO生物功能薄膜具有良好的血液相容性以及抗蛋白吸附性能。 关键词：RF-PECVD；PEO-like；血液相容性； 蛋白吸附 PEO-like film polymerization by RF-PECVD and its adsorption of protein Abstract：The paper presents the PEO-like functional film was polymerized by plasma-enhanced vapor chemical deposition (PECVD) on P-Si(100) surface with precursor EGDME and Ar in continuous wave (CW) and pulse plasma mode. We explored the effect of plasma parameter such as the discharge power, working pressure, the discharge mode on the film properties. The structure of coatings was analysed by Fourier transform infrared (FTIR) spectroscopy, contact angle measurement and Atomic Force Microscope (AFM). And we obtain that the lower power in CW or the longer Toff in pulsed plasma is contribute to more density of EO on the surface. At the same time, this paper also studied in vitro the adsorption of platelet-rich plasma (PRP) and protein cells, and observed the configuration of attached cell by invert microscope. We concluded that the plasma polymerization PEO-like functional film with outstanding structure and performance, can either in the smaller power CW, or in the longer Toff pulsed plasma mode, in which the PEO-like functional films demonstrate well blood compatibility and anti-protein adsorption performance. Keywords: RF-PECVD PEO-like blood compatibility protein adsorption * chenqiang@bigc.edu.cn Tel: 010-6026-1099 Fax: 010-6026-1108 1 前言 聚乙烯氧(PEO)具有较好的化学稳定性，它既耐酸又耐碱，具有广泛的应用范围[1]。它毒性低、具有高亲水性和高流动性，呈现出良好的生物惰性,可阻抗多种蛋白质和血细胞的粘附, 是一种改善高分子材料血液相容性的理想材料[2]。用等离子体放电的方式聚合材料/薄膜具有表面无针孔、膜质均匀