旱生植物内生细菌的分离及耐旱菌株的筛选鉴定-食品科学.PDF

124 2012, Vol. 33, No. 05 食品科学 ※生物工程 1 1 , 2 1 1 1 1 1 , 张彦涛 ，生吉萍 ，葛 佳 ，矫玉翠 ，王正荣 ，程凡升 ，申 琳 * (1. 中国农业大学食品科学与营养工程学院，北京 100083 ；2. 中国人民大学农业与农村发展学院，北京 100872) 为筛选耐旱内生细菌，并对其进行鉴定，以典型耐旱植物为材料，分离纯化出 142 株内生细菌。采用 液体培养基中添加聚乙二醇 6000 降低渗透势的方法分析内生细菌的耐旱情况。从中筛选出 1 株能够耐受质量浓度为 60g/100mL 聚乙二醇 6000 的内生细菌PB14 。对该菌株进行形态学观察，生理生化和 16S rDNA 序列鉴定，判定菌 株 PB14 为蜡样芽孢杆菌(Bacillus cereus) 。 耐旱；内生细菌；鉴定；蜡样芽孢杆菌 Isolation and Identification of Drought-tolerant Bacteria from Xerophtes 1 1,2 1 1 1 1 1, ZHANG Yan-tao ，SHENG Ji-ping ，GE Jia ，JIAO Yu-cui ，WANG Zheng-rong ，CHENG Fan-sheng ，SHEN Lin * (1. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China； 2. School of Agricultural Economics and Rural Development, Renmin University of China, Beijing 100872, China) Abstract：Drought-tolerant bacteria were isolated and identified in this paper. One hundred and forty two bacterial strains were isolated from the typical drought-tolerant plants. Through adding polyglycol 6000 (PEG6000) to the fluid nutrient medium for decreasing osmotic potential, the drought tolerance of endophytic bacteria were analyzed. One of these strains, PB14, has the capability of drought resistance up to 60 g/100 mL PEG6000. This strain was identified as Bacillus cereus based on morphological and biochemical experiments as well as 16S rDNA sequence analysis. Key words ：drought tolerance ；endophytic bacteria ；identification ；Bacillus cereus 中图分类号：TS201.3 文献标识码：A 文章编号：1002-6630(2012)05-0124-05 植物内生菌(endophyte)是指在其生活史的一部分或 类面临的严重生态问题，干旱已成为制约农