三角帆蚌肠道壁的乳酸菌的分离鉴定.doc

本科生毕业论文(设计) 题 目： 三角帆蚌肠道壁中乳酸 菌的初步分离与鉴定 姓名：贾迪 目 录 摘 要 4 关键词 4 前 言 5 1 实验材料 6 1.1实验动物 6 1.2 实验菌种 6 1.3 培养基 6 1.4 实验仪器及药品 6 2 实验方法 6 2. 1 蚌肠道壁中乳酸菌的分离… 7 2. 1.1 河蚌肠道壁总悬液的制备及稀释 7 2. 1.2 蚌肠道壁总悬液菌群培养 8 2. 1.3 蚌肠壁菌群的划线纯化 8 2. 1.4 已纯化菌的扩大培养 8 2. 2 蚌肠道壁中乳酸菌的分离… 9 2. 2.1 已纯化菌菌落观察及革兰氏染色鉴定 9 2. 2.2 已纯化菌的脱脂乳试管凝固鉴定 9 2. 2.3 已纯化菌BCG牛乳平板产酸鉴定 9 2. 2.4 已纯化菌糖发酵产酸产气鉴定 10 3实验结果及分析讨论 10 3. 1实验结果及讨论… 10 3.1.1菌落观察及菌体染色结果及讨论 10 3.1.2 脱脂乳试管凝固实验结果及讨论 11 3.1.3 产酸实验结果及讨论 11 3.1.4 产酸产气实验结果及讨论 11 3. 2分析讨论… 12 参考文献 12 致谢 13 三角帆蚌肠道壁中乳酸菌 的初步分离与鉴定 摘要 利用微生物学实验从河蚌肠道壁中分离纯化得到五株细菌（以下标号均为17-21号），并对这五株细菌进行初步鉴定实验。对这五株细菌进行革兰氏染色发现这五株菌染色结果均为蓝紫色，即为革兰氏阳性菌（G+）,且菌体单个散在。将这五株菌接种于BCG牛乳平板培养基，结果显示这五株细菌菌落周围培养基的pH值发生改变，且变为酸性。脱脂乳凝固实验中，这五株菌均能对脱脂乳进行凝固。产酸产气实验结果显示这五株菌均能产生酸性物质，使培养基的pH值变小，五株菌分解葡萄糖和蔗糖均不产气。经过初步鉴定实验结果显示可能为乳酸菌，是否确定为乳酸菌还需要后续的生化实验进一步鉴定。 关键词 三角帆蚌 乳酸菌 分离鉴定 The Preliminary separation and identification of lactobacillus in Bowel wall of Its jib clam Biological Sciences Class 07102 Jia di Instruct Teacher Li Na ABSTRACT Use microbiology test from HeBeng bowel wall of purification got five strains of bacteria(The following label all be 16-21), and the five strains of bacteria identified preliminarily tests. These five strains of bacteria found grams staining of these five strains bacteria dyeing results for violet, namely for Gram-positie organisms（G+）,and bacteria in a single scattered. Put this five strains of bacteria vaccination in BCG milk flat medium, the results showed that five strains bacterial colonies around the pH has changed，and become acidic。In the experimental of Skim milk solidification，these five strains of bacteria to skim milk has both frozen effect. The produces acid and gas experimental results show that the five strains of bacteria can produce acidity material，make medium pH value decreases，and five strains of bacteria decomp-osition glucose and sucrose can not producing gas. After a preliminary identification test results indicate that it may be for lactoba