基于串联质谱的快速肽段鉴定算法.PDF

36 18 2010 9 Vol.36 No.18 Computer Engineering September 2010 ·· 2010 A TP391 1, 2 1, 2 1, 2 1, 2 (1. 230026 2. 230026) RT-PSM FPI-PSMKeller RT-PSM FPI-PSM 5 13.6 ms 5.6 ms Fast Peptide Identification Algorithm Based on Tandem Mass Spectrum SHAO Ming-zhi1, 2, XU Yun1, 2, WANG Ying1, 2, LI Wen-jun1, 2 (1. Department of Computer Science and Technology, University of Science and Technology of China, Hefei 230026, China; 2. Anhui Province Key Laboratory of High Performance Computing, Hefei 230026, China) AbstractBased on existing RT-PSM algorithm, this paper proposes a new peptide identification algorithm called FPI-PSM, which transforms the digestion of protein into peptide database and proposes an optimization strategy for effective peak extraction. Ignoring post-translational modification, it conducts tests on the recognized Keller dataset. Compared to RT-PSM, FPI-PSM improves the sensitivity by 5, and decreases the average time of identifying each mass spectra from 13.6 ms to 5.6 ms. Key wordstandem mass spectrum; peptide identification; fragmentions; effective peak extraction 1 2 2.1 [1] (CID) 1 / N C CID N ab c C x y z b y 1 b1 y n-1 3 S