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E通过蘑菇多糖蛋白质复合物诱导免疫调节细胞因子的基因表达的小鼠
Life Scienca, Vol. 58, NO.21, Pp. 1755lSO3,1996
copyright 0 19%Ekvier science Inc.
Printed in the USA. All rights reserved
arm-32Qy96 $15.00 t .m
ELSEVIER PII SOO24-3205(96)00163-4
INDUCTION IN THE MOUSE OF GENE EXPRESSION OF IMMUNOMODULATING
CYTOKINES BY MUSHROOM POLYSACCHARIDE-PROTEIN COMPLEXES
F. Liu, M. C. Fung, V. E. C. Ooi and S. T. Chang
Department of Biology, The Chinese University of Hong Kong,
Shatin, Hong Kong
(Received in final form March 19,1996)
Two antitumor polysaccharide-protein complexes, PSPC and PSK from
mushrooms, were compared for their modulating effect on cytokine and cytokine
receptor gene expression. RNA samples were isolated from the splenocytes and
peritoneal exudate cells of the untreated or treated mice. Reverse transcription-
polymerase chain reaction (RT-PCR) was used to analyze the cytokine gene
expression. Nine out of 17 cytokine mRNAs and 5 out of 6 cytokine receptor
mRNAs were detected in the splenocytes and peritoneal exudate cells from both
untreated and tre
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