海藻 脂肪酸 分离.pdf

Analytica Chimica Acta 409 (2000) 257–266 Isolation and identification of unsaturated fatty acid methyl esters from marine micro-algae C. Viron ab , A. Saunois b , P. André b , B. Perly c , M. Lafosse a a Institut Chimie Organique et Analytique, CNRS UPRES A 6005, UFR Sciences, Université d’Orléans, BP 6759, 45067 Orléans Cedex 2, France b Laboratoire Substances Naturelles, Parfums Christian Dior, 45804 St. Jean de Braye Cedex, France c Service Chimie Moléculaire, C. E. N., 91191, Gif-sur-Yvette Cedex, France Received 18 July 1999; received in revised form 8 November 1999; accepted 14 November 1999 Abstract In order to study the biological activity of a micro-algae extract rich in fatty acids, the purification and structural identification of two hexadecadienoic methyl esters (16 : 2) and one hexadecatrienoic methyl ester (16 : 3) were performed. The purification of these molecules was carried out by liquid chromatography using a porous graphitic carbon phase. Determination of the chain length, degree and position of unsaturations was achieved by gas chromatography/electron impact mass spectrometer on dimethyloxazoline derivatives. With regard to the double bonds cis or trans isomery was determined by Fourier transform infrared spectroscopy. Structures were confirmed by 1H and 13C nuclear magnetic resonance. ©2000 Elsevier Science B.V. All rights reserved. Keywords: Porous graphitic carbon phase; Semi-preparative liquid chromatography; Hexadecatrienoic acid methyl ester; Hexadecadienoic acid methyl ester; Micro-alga 1. Introduction the cosmetic and pharmaceutical industries such as